A quick, convenient and economical method for the reliable determination of methylglyoxal in millimolar concentrations: the N-acetyl-L-cysteine assay

Analytical and Bioanalytical Chemistry
Rebekka WildGerald Münch

Abstract

The determination of methylglyoxal (MG) concentrations in vivo is gaining increasing importance as high levels of MG are linked to various health impairments including complications of diabetes. In order to standardize the measurements of MG in body fluids, it is necessary to precisely determine the concentration of MG stock solutions used as analytical standards. The "gold standard" method for the determination of MG concentration in the millimolar range is an enzyme-catalyzed endpoint assay based on the glyoxalase I catalyzed formation of S-lactoylglutathione. However, as this assay used purified glyoxalase I enzyme, it is quite expensive. Another method uses a derivation reaction with 2,4-dinitrophenylhydrazine, but this substance is explosive and needs special handling and storage. In addition, precipitation of the product methylglyoxal-bis-2,4-dinitrophenylhydrozone during the reaction limits the reliability of this method. In this study, we have evaluated a new method of MG determination based on the previously published fast reaction between MG and N-acetyl-L-cysteine at room temperature which yields an easily detectable condensation product, N-α-acetyl-S-(1-hydroxy-2-oxo-prop-1-yl)cysteine. When comparing these three di...Continue Reading

References

Dec 1, 1975·Analytical Chemistry·R P Gilbert, R B Brandt
Jan 1, 1995·Annual Review of Medicine·M Brownlee
Jan 1, 1993·Molecular Aspects of Medicine·P J Thornalley
Feb 15, 1993·European Journal of Biochemistry·S A Phillips, P J Thornalley
Jul 20, 1999·Chemical Research in Toxicology·G P FergusonY Al-Abed
Jun 2, 2009·The Journal of Physical Chemistry. a·Hadley E KriznerJeremy Kua
Aug 21, 2010·Journal of Food Science·J Wang, T Chang
Dec 14, 2011·The Journal of Biological Chemistry·Zhenyou ZouKe Zen

❮ Previous
Next ❯

Citations

Apr 13, 2013·Free Radical Biology & Medicine·Philip E MorganMichael J Davies
Jun 15, 2016·Molecular and Biochemical Parasitology·Praachi TiwariAmbak Kumar Rai
Aug 6, 2016·Environmental Science and Pollution Research International·Kamrun NaharMasayuki Fujita
Oct 13, 2016·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Anisur RahmanMasayuki Fujita
Feb 20, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Chun-Tao YangHui Zhang
Jun 16, 2015·Scientific Reports·Mohammad Golam MostofaLam-Son Phan Tran
Jun 19, 2017·Ecotoxicology and Environmental Safety·Jubayer Al MahmudMasayuki Fujita
May 4, 2017·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Usmangani AttarAltafhusain Nadaf
Jun 19, 2020·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·M Iqbal R KhanNafees A Khan
Nov 15, 2018·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Mirza HasanuzzamanMasayuki Fujita
Mar 27, 2018·Bio-medical Materials and Engineering·Gessica GiustoMarco Gandini
Jun 6, 2020·Frontiers in Plant Science·Jacob R MacWilliamsIsgouhi Kaloshian

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.