A quick guide to light microscopy in cell biology

Molecular Biology of the Cell
Kurt Thorn

Abstract

Light microscopy is a key tool in modern cell biology. Light microscopy has several features that make it ideally suited for imaging biology in living cells: the resolution is well-matched to the sizes of subcellular structures, a diverse range of available fluorescent probes makes it possible to mark proteins, organelles, and other structures for imaging, and the relatively nonperturbing nature of light means that living cells can be imaged for long periods of time to follow their dynamics. Here I provide a brief introduction to using light microscopy in cell biology, with particular emphasis on factors to be considered when starting microscopy experiments.

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Citations

Apr 1, 2017·Molecular Biology of the Cell·Kurt Thorn
Mar 16, 2018·Chemical Society Reviews·David P BishopDominic J Hare
Jun 29, 2018·Molecular Biology of the Cell·Jen-Yi Lee, Maiko Kitaoka
Jan 25, 2020·PloS One·Venera WeinhardtCarolyn A Larabell
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Sep 10, 2020·Nature Communications·Laura I FitzGeraldAngus P R Johnston
May 3, 2019·Journal of Biological Engineering·Qinfeng WuJeremiah J Zartman
Apr 1, 2020·Applied Optics·Mojtaba FalahatiLei Li
Sep 19, 2020·The International Journal of Biostatistics·Zahra Amini Farsani, Volker J Schmid
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May 24, 2021·Advanced Drug Delivery Reviews·Neha KapateSamir Mitragotri
Aug 27, 2021·Current Protocols·Jessica Valli, Jeremy Sanderson

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Methods Mentioned

BETA
light microscopy
Fluorescence microscopy
confocal microscopy
Light
Confocal

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