A rabbit reticulocyte model for the role of hemin-controlled repressor in hypochromic anemias

The Journal of Clinical Investigation
M L Freedman, J Rosman

Abstract

Hemin allows maximal protein synthesis in intact rabbit reticulocytes and their cell-free lysate preparations by retarding the formation of a translational repressor (HCR) found in the postribosomal supernate. In order to evaluate the role of HCR in the pathogenesis of hypochromic anemias, HCR was isolated and partially purified from intact rabbit reticulocytes incubated in vitro with either 0.1 mM alpha,alpha-dipyridyl (an iron-chelating agent) or 0.1 M ethanol. Both of these agents inhibit reticulocyte protein synthesis. Hemin (50 muM) protects against the inhibition by both agents. A ferrous iron-transferrin mixture, however, protects only against alpha,alpha-dipyridyl. Both alpha,alpha-dipyridyl and ethanol inhibit heme synthesis before the time that protein synthesis is affected, while neither lowers either ATP or GSH levels. These results indicate that while both agents inhibit heme synthesis, alpha,alpha-dipyridyl does so by inducing iron deficiency while ethanol works at a non-iron-requiring step. When HCR was isolated from intact cells and assayed in the reticulocyte cell-free systems, plus and minus hemin, premature appearance of HCR was found in cells incubated in vitro with alpha,alpha-dipyridyl or ethanol. When hem...Continue Reading

References

Jul 1, 1975·British Journal of Haematology·M L FreedmanF J Forte
Apr 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·Y BeuzardI M LONDON
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·M Gross, M RABINOVITZ
Jan 31, 1973·Nature: New Biology·S LegonT Hunt
May 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·H F Lodish
Aug 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M J ClemensI M LONDON
Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·C RaffelR Kaempfer
Nov 29, 1974·Annals of the New York Academy of Sciences·B H KaplanG Vanderhoff
Sep 18, 1973·Biochemical and Biophysical Research Communications·M L Freedman, S Karpatkin
May 28, 1972·Journal of Molecular Biology·T HuntI M LONDON
Mar 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·A I GrayzelI M LONDON
Feb 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·W V Zucker, H M Schulman
Jul 16, 1970·Biochimica Et Biophysica Acta·G A HowardE Herbert
May 1, 1968·Archives of Biochemistry and Biophysics·S D AdamsonW Godchaux
Apr 10, 1969·Biochemical and Biophysical Research Communications·C R Maxwell, M RABINOVITZ
Sep 26, 1967·Biochimica Et Biophysica Acta·H S WAXMANM RABINOVITZ
Jan 18, 1965·Biochemical and Biophysical Research Communications·G P BRUNS, I M LONDON
Jan 18, 1965·Biochemical and Biophysical Research Communications·D KARIBIAN, I M LONDON
May 3, 1965·Biochemical and Biophysical Research Communications·H S WAXMAN, M RABINOVITZ

Citations

Apr 23, 1979·Archives of Toxicology·R C SrivastavaK P Pandya
Mar 28, 1980·Biochimica Et Biophysica Acta·D L MarcusM L Freedman
Jan 1, 1982·Experimental Gerontology·D L MarcusM L Freedman
Jan 1, 1989·Toxicology in Vitro : an International Journal Published in Association with BIBRA·T GirbesP Alonso
Mar 1, 1979·British Journal of Haematology·L R Solomon, R S Hillman
Aug 1, 1988·Neurochemical Research·S TewariE W Fleming
Aug 1, 1986·Journal of the American Geriatrics Society·D L Marcus, M L Freedman
Jun 1, 1982·Environmental Research·D L DresnerR D Levere
Jan 1, 1981·Alcoholism, Clinical and Experimental Research·J W Ostroff

Related Concepts

Adenosine Triphosphate, Chromium Ammonium Salt
Ethanol
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Heme
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Reticulocytes

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