A RAC protein-binding site in the internal transcribed spacer 2 of Pre-rRNA transcripts from Schizosaccharomyces pombe.

The Journal of Biological Chemistry
Priyanka D AbeyrathneR N Nazar

Abstract

The interdependence of steps in the processing of the eukaryotic preribosomal rRNA transcripts indicate that rRNA processing, at least in part, acts as a quality control mechanism to help ensure that only functional rRNA is incorporated into mature ribosomes. In search of structural components that underlie this interdependence, we have isolated a large protein complex or RAC that contains an independent binding site for all four of the transcribed spacers in the nascent pre-rRNA. In this study the RAC-binding site in the internal transcribed spacer 2 sequence of Schizosaccharomyces pombe rRNA transcripts was identified, and the influence of this site on rRNA maturation was assessed. Modification exclusion analyses indicate that the protein complex interacts with a helical domain previously shown to contain features common to both the internal transcribed spacer 1 and the 3'-external transcribed spacer. Mutagenic analyses in vitro confirm an interaction with this sequence, and parallel analyses in vivo indicated a critical role in both the maturation of the rRNA components of the large subunit as well as the 18 S rRNA component of the small subunit. Hybridization analyses also indicated greatly elevated levels of unprocessed na...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D A Peattie
Sep 25, 1992·Nucleic Acids Research·L Good, R N Nazar
Aug 20, 1992·Journal of Molecular Biology·D I Van Ryk, R N Nazar
Feb 20, 1992·Journal of Molecular Biology·C A van der SandeR J Planta
May 23, 1969·Science·O L Miller, B R Beatty
Jan 1, 1980·Methods in Enzymology·G Chaconas, J H van de Sande
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D A Peattie, W Herr
May 1, 1983·Molecular Biology Reports·J P BachellerieF Raynal
Nov 24, 1995·The Journal of Biological Chemistry·Y LeeR N Nazar
Jun 3, 1994·Journal of Molecular Biology·Y F MelekhovetsR N Nazar
Feb 12, 1998·Journal of Molecular Biology·L GoodR N Nazar
Mar 29, 2000·Nucleic Acids Research·C AllmangD Tollervey
Mar 30, 2001·The Journal of Biological Chemistry·A I Lalev, R N Nazar

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