A radical switch in clonality reveals a stem cell niche in the epiphyseal growth plate

Nature
P T NewtonAndrei S Chagin

Abstract

Longitudinal bone growth in children is sustained by growth plates, narrow discs of cartilage that provide a continuous supply of chondrocytes for endochondral ossification1. However, it remains unknown how this supply is maintained throughout childhood growth. Chondroprogenitors in the resting zone are thought to be gradually consumed as they supply cells for longitudinal growth1,2, but this model has never been proved. Here, using clonal genetic tracing with multicolour reporters and functional perturbations, we demonstrate that longitudinal growth during the fetal and neonatal periods involves depletion of chondroprogenitors, whereas later in life, coinciding with the formation of the secondary ossification centre, chondroprogenitors acquire the capacity for self-renewal, resulting in the formation of large, stable monoclonal columns of chondrocytes. Simultaneously, chondroprogenitors begin to express stem cell markers and undergo symmetric cell division. Regulation of the pool of self-renewing progenitors involves the hedgehog and mammalian target of rapamycin complex 1 (mTORC1) signalling pathways. Our findings indicate that a stem cell niche develops postnatally in the epiphyseal growth plate, which provides a continuous ...Continue Reading

References

Apr 13, 1995·The New England Journal of Medicine·E K Rowinsky, R C Donehower
Mar 16, 2006·Toxicologic Pathology·Anthony P HallPeter F Wadsworth
Aug 9, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Eiichiro NakamuraSusan Mackem
Apr 6, 2007·Proceedings of the National Academy of Sciences of the United States of America·Yukiko MaedaBeate Lanske
Feb 15, 2008·Proceedings of the National Academy of Sciences of the United States of America·Adrian Salic, Timothy J Mitchison
Apr 28, 2010·Laboratory Investigation; a Journal of Technical Methods and Pathology·Carlos E de AndreaPancras C W Hogendoorn
Dec 24, 2010·Nature·Shomit SenguptaDavid M Sabatini
May 5, 2011·Hormone Research in Pædiatrics·Joyce EmonsJan M Wit
Oct 11, 2011·Cell Stem Cell·Toshio SudaGregg L Semenza
Sep 3, 2013·Nature·Madeline A LancasterJuergen A Knoblich
Sep 24, 2013·Nature Methods·Simone PicelliRickard Sandberg
Jan 5, 2014·Nature Protocols·Simone PicelliRickard Sandberg
May 20, 2014·Nature Methods·Peter V KharchenkoDavid T Scadden
Aug 8, 2014·Nature Medicine·Ya-Chieh HsuElaine Fuchs
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
May 28, 2016·Science·Panteleimon RompolasValentina Greco
Jul 9, 2016·Nature Communications·Susanne NichterwitzEva Hedlund
Aug 6, 2016·Science Advances·Marketa KauckaIgor Adameyko
Jan 24, 2018·Stem Cell Reports·Oleg PodgornyGrigori Enikolopov

❮ Previous
Next ❯

Citations

Jun 11, 2019·Integrative and Comparative Biology·Kimberly L Cooper
Mar 7, 2019·Nature Reviews. Endocrinology·Claire Greenhill
Oct 2, 2019·Frontiers in Cell and Developmental Biology·Thomas H AmbrosiCharles K F Chan
Mar 10, 2019·Nature·Manuela Wuelling, Andrea Vortkamp
May 22, 2020·Wiley Interdisciplinary Reviews. Developmental Biology·Brett J Kagan, Alberto Rosello-Diez
Nov 5, 2019·Frontiers in Genetics·Jessica Cristina Marín-LleraJesús Chimal-Monroy
Aug 6, 2020·Frontiers in Cell and Developmental Biology·Anastasiia D KurenkovaAndrei S Chagin
Mar 17, 2020·Current Osteoporosis Reports·Yuki MatsushitaNoriaki Ono
Mar 13, 2020·Development·Maxwell A SerowokyFrancesca V Mariani
Dec 5, 2019·International Journal of Molecular Sciences·Shawn A HallettNoriaki Ono
Sep 29, 2020·Frontiers in Cell and Developmental Biology·Sophia Ka Yan MaDanny Chan
Jul 10, 2019·Stem Cells International·Jun-Qi LiuZhen Tan
Sep 22, 2020·European Journal of Pain : EJP·Larissa de ClauserShafaq Sikandar
Nov 1, 2020·Science Advances·Fei FangStavros Thomopoulos
Dec 12, 2020·Current Osteoporosis Reports·Hongyuan Zhang, Benjamin A Alman
Dec 23, 2020·Proceedings. Biological Sciences·Petra FrýdlováDaniel Frynta
Jan 6, 2021·American Journal of Medical Genetics. Part a·Lorenzo D BottoAndrea Superti-Furga
Dec 29, 2020·Experimental Physiology·Elaine Zhou, Julian Lui
Jan 13, 2021·Advanced Healthcare Materials·Calvin ChangHai-Quan Mao
Dec 29, 2020·Experimental Cell Research·Sarah HozainJessica Cottrell
Jan 23, 2021·International Journal of Molecular Sciences·Ángela Fernández-IglesiasJosé Manuel López
Mar 5, 2021·Science Translational Medicine·Takamitsu MaruyamaWei Hsu
Mar 27, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Francesca Manuela Johnson de Sousa BritoAnna Daroszewska
Oct 18, 2020·Molecular and Cellular Endocrinology·Manisha DixitShoshana Yakar
Apr 11, 2021·Nature Reviews. Molecular Cell Biology·Alexander Rauch, Susanne Mandrup
Mar 17, 2021·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Eiki KoyamaMaurizio Pacifici
Jun 27, 2021·Proceedings of the National Academy of Sciences of the United States of America·Prem Swaroop YadavYingzi Yang
Jun 22, 2021·Frontiers in Bioengineering and Biotechnology·Xianggang WangJincheng Wang
Jul 3, 2021·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Toshihide Mizoguchi, Noriaki Ono
Jul 20, 2021·ELife·Thomas H AmbrosiCharles Kf Chan
Aug 5, 2021·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Nora E RenthalJoel N Hirschhorn
Aug 7, 2021·Emerging Topics in Life Sciences·David Kuntin, Paul Genever
Aug 13, 2021·Nature·Thomas H AmbrosiCharles K F Chan
Sep 29, 2021·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Sara P AbrahamMichaela Bosakova
Nov 6, 2021·Development·Hae Ryong KwonLorin E Olson

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Datasets Mentioned

BETA
GSE113982

Methods Mentioned

BETA
laser-capture microdissection
fluorescence-activated cell sorting
PCR
confocal microscopy
FACS
dissection
RNA-seq

Software Mentioned

STAR
PAGODA
ENSEMBL
TreeStar
Excel
FeatureCounts
GraphPad Prism
Oriana
Illumina Casava
DESeq2

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