PMID: 5901503Jan 1, 1966

A radioautographic study with H3-thymidine on adrenal medulla nuclei of rats intermittently exposed to cold

The Journal of Cell Biology
M P Viola-Magni

Abstract

A considerable decrease (24 to 40%) of DNA content per nucleus previously observed in the adrenal medulla of rats exposed intermittently to cold is followed by restoration to normal and supranormal values. This phenomenon has now been studied by use of H(3)-thymidine, which was given to normal rats, to rats exposed to cold, and to animals brought to room temperature after cold exposure. In the first two conditions, no significant labeling of nuclei was observed. In the third, labeling took place clearly in the 1st 3 days. The grain counts showed that the early labeled nuclei had more grains than those labeled later, indicating differences in the rate of DNA synthesis. A statistically significant correlation was found, on the same nuclei, between amount of Feulgen dye and number of grains. It is concluded that net synthesis of DNA takes place in the phase of recovery from cold. This fact is not related to cell division, as no mitoses could ever be detected, but rather to the cold-induced loss of DNA. Clear demonstration is thus given of a marked variation in the amount of DNA per nucleus in relation to the functional conditions of adrenal medulla cells.

References

Apr 1, 1958·Experimental Cell Research·S R Pelc
Nov 25, 1958·The Journal of Biophysical and Biochemical Cytology·H KOENIG
Jun 1, 1959·Experimental Cell Research·H QUASTLER, F G SHERMAN
Sep 10, 1960·Nature·L G LAJTHAE HELLE
Jul 1, 1960·The Journal of Biophysical and Biochemical Cytology·J G GALL, W W JOHNSON
Jul 1, 1964·The Journal of Cell Biology·S R Pelc

Citations

Jan 1, 1975·Histochemistry·C Bibbiani, M P Viola-Magni
May 15, 1968·Experientia·G MalvaldiM P Viola-Magni
Mar 1, 1968·Experimental Cell Research·U A Sod-Moriah, G H Schmidt
Jul 13, 1968·Nature·S R Pelc
Aug 1, 1968·The New England Journal of Medicine·J Post, J Hoffman
Aug 1, 1969·The Journal of Cell Biology·S R Pelc, M P Viola-Magni
Jun 27, 1969·Biochemical and Biophysical Research Communications·P J O'Connor
Jan 1, 1970·Histochemie. Histochemistry. Histochimie·D Hopwood
Oct 1, 1972·Journal of Microscopy·M P Viola-Magni

Related Concepts

Adrenal Medulla
Autoradiography
Cell Division Phases
Cell Nucleus
Cold Temperature
DNA, Double-Stranded
Photometry
Thymidine
Tritium

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