PMID: 7026682Jan 1, 1981Paper

A radioimmunoassay for determination of anti-actin antibodies

Journal of Immunological Methods
R ThorstenssonA Fagraeus

Abstract

The reaction of spontaneously occurring human anti-human antibodies and experimentally produced rabbit anti-actin antibodies was investigated in a solid-phase radioimmunoassay (RIA). Three structurally different in vitro forms of actin monomeric G-actin filamentous F-actin and aggregated denatured actin were used as antigens. Human anti-actin antibodies reacted with F- and G-actin but not with aggregated actin, while rabbit anti-actin antibodies gave a strong reaction with all 3 forms of actin indicating differences in antibody specificities. The results of the anti-actin RIA were compared with those obtained by indirect immunofluorescence (IFL) on cryostat sections of rat stomach. The anti-actin RIA discriminated between patients' sera and control sera in most cases, although the indirect IFL test gave more conclusive results. The seemingly low sensitivity of the anti-actin RIA compared with that of indirect IFL test for detection of human anti-actin antibodies is probably due to favourable antigen distribution in tissue, not available in the solid phase. The anti-actin RIA was able to detect anti-actin antibodies in 8 out of 8 immunized rabbits although only two produced antibodies detectable by indirect IFL. The differences ...Continue Reading

References

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Citations

Mar 16, 1984·Journal of Immunological Methods·P Kurki, I Virtanen
Nov 20, 1986·Journal of Immunological Methods·W L Hoffman, A A Jump
Jan 1, 1982·Journal of Immunological Methods·R NorbergG Utter
Aug 18, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·Charline Wehlou, Joris R Delanghe
Jan 1, 1994·Clinical and Diagnostic Laboratory Immunology·P W FrenchJ L Yang

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