A random-sequential mechanism for nitrite binding and active site reduction in copper-containing nitrite reductase.

The Journal of Biological Chemistry
Hein J WijmaGerard W Canters

Abstract

The homotrimeric copper-containing nitrite reductase (NiR) contains one type-1 and one type-2 copper center per monomer. Electrons enter through the type-1 site and are shuttled to the type-2 site where nitrite is reduced to nitric oxide. To investigate the catalytic mechanism of NiR the effects of pH and nitrite on the turnover rate in the presence of three different electron donors at saturating concentrations were measured. The activity of NiR was also measured electrochemically by exploiting direct electron transfer to the enzyme immobilized on a graphite rotating disk electrode. In all cases, the steady-state kinetics fitted excellently to a random-sequential mechanism in which electron transfer from the type-1 to the type-2 site is rate-limiting. At low [NO(-)(2)] reduction of the type-2 site precedes nitrite binding, at high [NO(-)(2)] the reverse occurs. Below pH 6.5, the catalytic activity diminished at higher nitrite concentrations, in agreement with electron transfer being slower to the nitrite-bound type-2 site than to the water-bound type-2 site. Above pH 6.5, substrate activation is observed, in agreement with electron transfer to the nitrite-bound type-2 site being faster than electron transfer to the hydroxyl-bo...Continue Reading

References

Sep 30, 1992·Biochemical and Biophysical Research Communications·E Libby, B A Averill
May 29, 1987·Biochemical and Biophysical Research Communications·M A KashemJ LeGall
Mar 20, 1995·Analytical Biochemistry·A J Brenner, E D Harris
Mar 31, 1998·Biosensors & Bioelectronics·S SasakiT Beppu
May 1, 1999·Journal of Molecular Biology·R W StrangeS S Hasnain
May 20, 2000·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·E MonzaniJ Reedijk
Dec 1, 2000·Nature·H W Bange
Jul 27, 2001·Acta Crystallographica. Section D, Biological Crystallography·M J EllisS S Hasnain
Feb 28, 1996·Inorganic Chemistry·Luigi CasellaMassimiliano Frassoni
Nov 7, 1996·Chemical Reviews·Bruce A. Averill
Jan 23, 2003·Protein Science : a Publication of the Protein Society·Martin J Boulanger, Michael E P Murphy
May 6, 2004·Journal of the American Chemical Society·Antonietta Impagliazzo, Marcellus Ubbink
May 8, 2004·Science·Elitza I TochevaMichael E P Murphy
Jul 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·F E DoddB E Smith
Aug 11, 2004·Biochemistry·Hein J WijmaMartin Ph Verbeet
Jan 26, 2005·Biochemical Society Transactions·J D Rock, J W B Moir
May 19, 2005·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Yann AstierHein J Wijma

❮ Previous
Next ❯

Citations

May 24, 2012·Accounts of Chemical Research·Quan WangW E Moerner
Apr 20, 2010·Journal of the American Chemical Society·Armand W J W Tepper
Sep 24, 2011·The Journal of Physical Chemistry. B·Lukasz KrzemińskiLars J C Jeuken
Feb 28, 2008·Proceedings of the National Academy of Sciences of the United States of America·Sofya KuznetsovaGerard W Canters
Oct 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Randall H GoldsmithW E Moerner
Dec 14, 2012·Proceedings of the National Academy of Sciences of the United States of America·Matteo TegoniVincent L Pecoraro
Jul 10, 2009·The Journal of Biological Chemistry·Sibylle BrennerNigel S Scrutton
Mar 26, 2014·Chemical Reviews·Fangting YuVincent L Pecoraro
Mar 5, 2014·Chemical Reviews·Edward I SolomonLi Tian
May 16, 2007·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Konstantinos ParaskevopoulosS Samar Hasnain
Aug 23, 2008·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Isabel MouraJosé J G Moura
Oct 3, 2008·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Steven M BerryNicholas A Zehm
Aug 8, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Leandro C TabaresGerard W Canters
Mar 2, 2016·Proceedings of the National Academy of Sciences of the United States of America·Yohta FukudaEiichi Mizohata
Apr 8, 2016·Inorganic Chemistry·Steven M BerryAlexandra M Sauer
Aug 4, 2015·Accounts of Chemical Research·Catherine S Mocny, Vincent L Pecoraro
Apr 4, 2014·Chemical Reviews·Luisa B Maia, José J G Moura
Jul 14, 2017·Metallomics : Integrated Biometal Science·Sam HorrellMichael A Hough
Mar 16, 2018·Metallomics : Integrated Biometal Science·Masami Lintuluoto, Juha Mikael Lintuluoto
Mar 30, 2018·Chemical Society Reviews·O ZozuliaI V Korendovych
Jul 16, 2014·Nature Communications·Nicole G H LeferinkS Samar Hasnain
Jul 17, 2013·The Journal of Biological Chemistry·Thomas J LawtonAmy C Rosenzweig
Sep 15, 2020·Physical Chemistry Chemical Physics : PCCP·Ronny ChengBinju Wang
Nov 21, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kakali SenThomas W Keal
Feb 12, 2020·Proceedings of the National Academy of Sciences of the United States of America·Yohta FukudaTaro Tamada
Dec 20, 2017·World Journal of Microbiology & Biotechnology·Haofeng GaoNan Hu
Mar 21, 2009·Analytical Chemistry·Vincent FourmondChristophe Léger
Feb 19, 2010·The Journal of Physical Chemistry. B·Vincent FourmondChristophe Léger
Jan 4, 2007·Journal of the American Chemical Society·Antonietta ImpagliazzoMarcellus Ubbink
Jan 17, 2015·Biochemistry·Yan LiJ Bernholc

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.