A rapid analytical method for the quantitative determination of the sugar in acarbose fermentation by infrared spectroscopy and chemometrics

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Wanzhu ZhouYongqian Lei

Abstract

In this article, a rapid analytical method for the quantitative determination of the glucose and maltose in the industrial acarbose fermentation was established by the combination application of infrared spectroscopy and chemometrics. The spectra of the 398 acarbose samples were collected by a portable infrared fast analyzer and the concentration of the glucose and maltose in the acarbose fermentation solution were determinate by high performance liquid chromatography (HPLC) as the referent database. Four spectral pretreatment methods, first derivative (FD), second derivative (SD), Savitzky-Golay (SG) convolution smoothing and mean center (MC) were employed to eliminate the optical interference from background and other noise information. The best result was obtained with FD+SG(21, 3)+MC method. The effects of different principal component numbers (PCs) on the parameters were also optimized. Two models of PLS and MLR, were used to predict the concentration of the glucose and maltose. The FD+SG(21, 3)+MC method was chosen as best method, with 12 PCs for glucose and 11 for maltose as optimized parameters. The PLS model was significantly better than the MLR model. Furthermore, both the predicted values and the reference values of ...Continue Reading

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