Dec 24, 1975

A rapid and comprehensive system for the routine identification of drugs in biological material based on microphase extraction and drug colour profiles

Journal of Chromatography
W J SerfonteinL De Villiers

Abstract

The separation of basic, acidic and neutral drugs from propanol-2 extracts of serum, urine and tissue homogenates at different pH values using a micro-phase extraction technique is described. Following preliminary screening, the various drug-containing fractions obtained are further examined by two-dimensional thin-layer chromatography. The drugs present are identified with reference to documented standards with the aid of a drug colour profile system and RF values relative to three different reference standards. By means of gas chromatographic analysis of the same extracts, semi-quantitative estimates of the amounts of drugs present, which are sufficiently accurate for clinical emergency purposes, can be made in many instances. The main advantages of the system are "clean" extracts with a minimum of background interference, rapidity (4-6 h for a complete analysis) and systematically documented and visually presented behaviour of drugs after spraying with various chromogenic and fluorogenic reagents, allowing the systematic identification of unknown substances.

  • References3
  • Citations2

References

  • References3
  • Citations2

Citations

Mentioned in this Paper

Urine
Tissue Extracts
Systems Analysis
Pharmaceutical Preparations
Propanols
1-Propanol
Acids
Colorimetry
Urine - SpecimenType
Hydrogen-Ion Concentration

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