A rapid and economic in-house DNA purification method using glass syringe filters.

PloS One
Yun-Cheol Kim, Sherie L Morrison

Abstract

Purity, yield, speed and cost are important considerations in plasmid purification, but it is difficult to achieve all of these at the same time. Currently, there are many protocols and kits for DNA purification, however none maximize all four considerations. We now describe a fast, efficient and economic in-house protocol for plasmid preparation using glass syringe filters. Plasmid yield and quality as determined by enzyme digestion and transfection efficiency were equivalent to the expensive commercial kits. Importantly, the time required for purification was much less than that required using a commercial kit. This method provides DNA yield and quality similar to that obtained with commercial kits, but is more rapid and less costly.

References

Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Apr 1, 1991·Applied and Environmental Microbiology·G RomanowskiW Wackernagel
May 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·R RadloffJ Vinograd
Jun 1, 1981·Analytical Biochemistry·D S Holmes, M Quigley
Oct 10, 1998·Journal of Virology·T DullL Naldini

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Citations

Jun 6, 2018·PeerJ·Olga GiraldoOscar Corcho
Jul 1, 2017·3 Biotech·Obrador-Sánchez José AbrahamCanto-Canché Blondy
Mar 1, 2016·Functional Plant Biology : FPB·Tim WendlandtChristine Stöhr

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Methods Mentioned

BETA
transfection
electrophoresis

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