A Rapid and High Throughput MIC Determination Method to Screen Uranium Resistant Microorganisms

Methods and Protocols
Meenakshi AgarwalAshvini Chauhan

Abstract

The assessment of minimum inhibitory concentration (MIC) is a conventional technique used for the screening of microbial resistance against antibiotics, biocides, and contaminants such as heavy metals. However, as part of our ongoing work, we have observed biases associated with using traditional liquid MIC method to screen microbial heavy metal resistance, including both bacterial and fungal strains. Specifically, the addition of uranium into synthetic media causes immediate precipitation prior to the initiation of microbial growth, thus hampering the optical density measurements, and the obtained MIC values are thus flawed and inaccurate. To address this discrepancy, we report the optimization and development of a serial-dilution-based MIC method conducted on solid growth media supplemented with uranium, which is more accurate, relative to the testing of MICs performed in liquid cultures. Notably, we report on the efficacy of this method to screen not only bacteria that are resistant to uranium but also demonstrate the successful application to yeast and fungal isolates, for their ability to resist uranium, is more accurate and sensitive relative to the liquid method. We believe that this newly developed method to screen heav...Continue Reading

References

Jun 1, 1982·Journal of Bacteriology·K J Kwon-ChungT J Popkin
Oct 12, 2000·Clinical Microbiology Reviews·E S Jacobson
Jun 23, 2001·The Journal of Antimicrobial Chemotherapy·J M Andrews
Oct 13, 2001·Critical Reviews in Microbiology·N Durán, C F Menck
Oct 14, 2003·Biochemical Pharmacology·Ricardo Pérez-TomásVanessa Soto-Cerrato
Feb 6, 2004·British Medical Bulletin·Lars Järup
Jun 24, 2004·Infection and Immunity·Gee W LauDimitri Mavrodi
Oct 27, 2006·Antimicrobial Agents and Chemotherapy·Joshua D Nosanchuk, Arturo Casadevall
Nov 17, 2006·Nature Reviews. Microbiology·Neil R WilliamsonGeorge P C Salmond
Oct 17, 2007·Environmental Science & Technology·Andri Stefánsson
Sep 4, 2009·Trends in Microbiology·George Y Liu, Victor Nizet
Mar 22, 2012·Journal of Basic Microbiology·Rehab M Mohamed, Aly E Abo-Amer
Sep 6, 2012·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Benmalek YaminaFardeau Marie Laure
Nov 20, 2012·Current Opinion in Biotechnology·Kenneth H WilliamsDerek R Lovley
Sep 18, 2013·Inorganic Chemistry·Debbie C CransEugenijus Norkus
Jan 31, 2014·Journal of Environmental Health Science & Engineering·Hoda AlboghobeishMonir Doudi
Jun 26, 2015·Interdisciplinary Toxicology·Monisha JaishankarKrishnamurthy N Beeregowda
Oct 3, 2018·Coordination Chemistry Reviews·Manoj SaxenaArthur D Tinoco

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