A rapid and high-throughput microplate spectrophotometric method for field measurement of nitrate in seawater and freshwater

Scientific Reports
Jiapeng WuJiali Wen

Abstract

The well-known zinc-cadmium reduction method is frequently used for determination of nitrate. However, this method is seldom to be applied on field research of nitrate due to the long time consuming and large sample volume demand. Here, we reported a modified zinc-cadmium reduction method (MZCRM) for measurement of nitrate at natural-abundance level in both seawater and freshwater. The main improvements of MZCRM include using small volume disposable tubes for reaction, a vortex apparatus for shaking to increase reduction rate, and a microplate reader for high-throughput spectrophotometric measurements. Considering salt effect, two salinity sections (5~10 psu and 20~35 psu) were set up for more accurate determination of nitrate in low and high salinity condition respectively. Under optimized experimental conditions, the reduction rates were stabilized on 72% and 63% on the salinity of 5 and 20 psu respectively. The lowest detection limit for nitrate was 0.5 μM and was linear up to 100 μM (RSDs was 4.8%). Environmental samples assay demonstrated that MZCRM was well consistent with conventional zinc-cadmium reduction method. In total, this modified method improved accuracy and efficiency of operations greatly, and would be realize...Continue Reading

References

Aug 15, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wenjuan S JobgenGuoyao Wu
Oct 31, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Steve M Helmke, Mark W Duncan
Oct 31, 2008·Talanta·M J MoorcroftR G Compton
Feb 19, 2011·Annual Review of Marine Science·Phyllis Lam, Marcel M M Kuypers

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Citations

Dec 26, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Pheeraya JaikangKate Grudpan
Sep 21, 2018·Frontiers in Microbiology·Jiapeng WuXiaohan Liu
Feb 14, 2020·ACS Sensors·Wenyue GaoEric Bakker

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