PMID: 616042Apr 1, 1977

A rapid and precise sequential saturation radioimmunoassay for thyroxine

Scandinavian Journal of Clinical and Laboratory Investigation
V Kruse, O Lind

Abstract

A radioimmunoassay for the measurement of total thyroxine in unextracted serum or plasma is described. The assay is performed according to the sequential saturation principle in less than 4 h. The entire assay is carried out at room temperature. An antiserum of high affinity was used as binder, ammonium 8-anilino-1-naphthalene-sulphonate as displacing agent, and activated charcoal as adsorbent in the separation step. The standard curve was linear in the range 0-136 nmol/l serum. The precision in terms of SD was nearly the same through-out this range. Estimates of within-assay SD varied from 1.3 to 4.1 and between-assay SD from 0 to 3.3 nmol/l. The recovery of thyroxine added to normal plasma was 100% and the sensitivity was 6 nmol/l. Mean thyroxine concentration in plasma from young bulls was 92 nmol/l. The assay appears to be very well suited for experimental purposes when high precision is essential.

References

Apr 1, 1976·Clinica Chimica Acta; International Journal of Clinical Chemistry·J J Pratt, M G Woldring
Apr 2, 1975·Clinica Chimica Acta; International Journal of Clinical Chemistry·G S ChallandJ G Ratcliffe
Jan 1, 1976·Scandinavian Journal of Clinical and Laboratory Investigation·V Kruse
May 3, 1976·Clinica Chimica Acta; International Journal of Clinical Chemistry·J SethW J Irvine
Jan 1, 1974·British Medical Bulletin·R P Ekins
Mar 24, 1972·Biochemical and Biophysical Research Communications·T MitsumaC S Hollander

Citations

Oct 1, 1979·Scandinavian Journal of Clinical and Laboratory Investigation·V Kruse
Dec 1, 1979·Zeitschrift für Tierphysiologie, Tierernährung und Futtermittelkunde·S Rotenberg, P E Jakobsen
Jan 1, 1983·Critical Reviews in Clinical Laboratory Sciences·T D Trainer, P L Howard

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Binding Sites, Antibody
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