A rapid and repeatable method for venom extraction from cubozoan nematocysts

Toxicon : Official Journal of the International Society on Toxinology
T Carrette, Jamie Seymour

Abstract

Various comparative studies into the biological activity and relative toxicity of cubozoan venoms have been investigated, in particular the venom from the potentially lethal cubozoan Chironex fleckeri. Efficient and reliable extraction of venom from nematocysts is essential before any research into venom toxicity can be conducted and previous cited methods of extraction have varied greatly, each with their own associated problems. A new standardised technique for the recovery of venom from nematocysts of cubozoans is investigated to decrease the variation displayed between authors due to differing extraction techniques. The use of a mini bead mill beater, as investigated in this trial, allows for the rapid extraction of venom from nematocysts and is devoid of the previously isolated problems experienced with other methods of venom isolation, such as excessive heat build up.

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Citations

Apr 23, 2014·Toxicon : Official Journal of the International Society on Toxinology·Changkeun KangEuikyung Kim
Aug 28, 2014·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Athena AndreossoJamie E Seymour
Oct 7, 2014·Toxicon : Official Journal of the International Society on Toxinology·Sophie Badré
Aug 9, 2011·Journal of Pharmacological and Toxicological Methods·Changkeun KangEuikyung Kim
Feb 17, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Jinhua FengPengcheng Li
Jan 5, 2010·The Journal of Emergency Medicine·Isaac FernandezGeorge Sternbach
Dec 23, 2009·Toxicon : Official Journal of the International Society on Toxinology·Liang XiaoLi-ming Zhang
Jul 22, 2009·Toxicon : Official Journal of the International Society on Toxinology·Jinhua FengPengcheng Li
Mar 24, 2009·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Changkeun KangEuikyung Kim
Mar 4, 2009·Journal of Pharmacological and Toxicological Methods·Nicki KonstantakopoulosWayne C Hodgson
Jun 13, 2008·Toxicology Letters·Kelly L WinterWayne C Hodgson
Feb 5, 2008·Toxicon : Official Journal of the International Society on Toxinology·Diane Brinkman, James Burnell
Aug 11, 2007·Toxicon : Official Journal of the International Society on Toxinology·Diane Brinkman, James Burnell
Mar 31, 2007·Toxicon : Official Journal of the International Society on Toxinology·Avril H Underwood, Jamie E Seymour
Jan 12, 2007·Toxicon : Official Journal of the International Society on Toxinology·K L WinterW C Hodgson
Jul 21, 2015·Journal of Proteomics·Indu ChoudharyEuikyung Kim
Mar 1, 2005·Toxicon : Official Journal of the International Society on Toxinology·T Carrette, J Seymour
Feb 3, 2005·Toxicon : Official Journal of the International Society on Toxinology·Sharmaine RamasamyWayne C Hodgson
Apr 28, 2004·Toxicon : Official Journal of the International Society on Toxinology·Sharmaine RamasamyWayne C Hodgson
Aug 25, 2006·Toxicon : Official Journal of the International Society on Toxinology·James Tibballs
Oct 11, 2005·Bioorganic & Medicinal Chemistry Letters·Cuiping LiPengcheng Li
Sep 17, 2005·Toxicon : Official Journal of the International Society on Toxinology·Anna H KintnerSusan L Edwards
Jul 2, 2011·Toxicon : Official Journal of the International Society on Toxinology·Hyunkyoung LeeEuikyung Kim
Apr 15, 2016·Marine Drugs·Bárbara Frazão, Agostinho Antunes
Nov 2, 2013·Toxicon : Official Journal of the International Society on Toxinology·Peter Pereira, Jamie E Seymour
Jan 28, 2014·Toxicon : Official Journal of the International Society on Toxinology·Stephanie ChaousisJamie Seymour
May 9, 2012·Toxicon : Official Journal of the International Society on Toxinology·Silvia L A Saggiomo, Jamie E Seymour
Sep 27, 2012·Toxicon : Official Journal of the International Society on Toxinology·S McClounan, J Seymour
May 11, 2016·Toxicon : Official Journal of the International Society on Toxinology·Pamela Berilyn T SoOliver B Villaflores
Oct 30, 2016·Toxicon : Official Journal of the International Society on Toxinology·Yunwi HeoEuikyung Kim
Nov 7, 2016·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Fernando Lazcano-PérezJudith Sánchez-Rodríguez
Nov 9, 2016·Toxicon : Official Journal of the International Society on Toxinology·Yang YuePengcheng Li
May 1, 2008·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·A Garm, J Bielecki

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