PMID: 9000648Jan 1, 1997Paper

A rapid and sensitive quantitation method of endogenous morphine in human plasma

Life Sciences
Y LiuG B Stefano

Abstract

Endogenous morphine is found at very low levels (pg/ml) in human plasma. Without technical modification, the usual techniques, i.e., RIA or HPLC, can not directly detect its presence. As a result the initial purification process is very crucial. In this report we demonstrate this opiate alkaloids determination in 2 ml of human plasma. Following an acidified precipitation of proteins in plasma, a solid-phase extraction, Sep-pak collection, was employed to concentrate the endogenous morphine. The extract was dissolved in a pH 8.9 phosphate buffer and then extracted into an organic solvent (chloroform and isopropyl alcohol). The initially purified sample was separated by HPLC and detected with an electrochemical detector of 500 mV and 0.02 Hz. The results showed that the recovery of morphine by this technique was 86+5.7%, and the sensitivity was 50 pg/ml. Compared to the detection sensitivity of the HPLC (2 ng/ml), this method increased the detection limit by 40-fold. With this method the concentration of endogenous morphine in plasma was determined as 80 pg/ml.

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Citations

Aug 15, 2000·Trends in Neurosciences·G B StefanoE Bianchi
Feb 26, 1998·International Journal of Cardiology·V Brix-ChristensenG B Stefano
Jun 6, 2000·Brain Research. Molecular Brain Research·Y Goumon, G B Stefano
Jan 8, 1999·Brain Research. Molecular Brain Research·G B StefanoM Salzet
Aug 13, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·M R MoellerT Kraemer
May 22, 2009·Annals of the New York Academy of Sciences·Wei Zhu, George B Stefano
Jan 30, 2004·The Journal of Toxicological Sciences·Hideyuki YamadaKazuta Oguri
Jul 23, 2008·Journal of Neurochemistry·Carla NeriEnrica Bianchi
Jul 11, 2008·Journal of Clinical Pharmacy and Therapeutics·S O MashayekhiR D W Hain
Feb 17, 2009·Journal of Separation Science·Steen Honoré Hansen
Mar 24, 2010·The British Journal of Surgery·K M MadboulyC P Delaney
Sep 13, 2019·European Journal of Mass Spectrometry·D T UsmanovBSh Kasimov
Sep 9, 2008·Neurochemical Research·George B StefanoWei Zhu
Apr 12, 2000·European Journal of Biochemistry·V LaurentM Salzet
Nov 23, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wei ZhuGeorge B Stefano
Jul 27, 2000·Endothelium : Journal of Endothelial Cell Research·P CadetG B Stefano

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