A Rapid and Simple Method for Detection of Colchicum autumnale Using PCR

Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan
Akiko TeraiTakeo Sasamoto

Abstract

Colchicum autumnale is a perennial, toxic plant that originated in Europe and North Africa. Although inedible, it is occasionally consumed accidentally because it resembles the edible Allium victorialis and other related species. This misidentification has led to episodes of food poisoning in Japan. However, determining the causative agent of a food poisoning outbreak by observing the sample visually or analyzing the chemical composition is challenging when dealing with small samples. Therefore, we developed a novel set of PCR primers that anneal to the internal transcribed spacer (ITS) region of C. autumnale ribosomal DNA, designed to detect the presence of C. autumnale in small samples. These primers successfully detected C. autumnale in all samples in which it was present, and did not give a positive PCR band in the 48 other distinct crop species tested, in which it was not present. Further, our method could amplify DNA from samples of C. autumnale that had been heat-treated and digested using artificial gastric fluids. Thus, this PCR strategy is highly specific and can be used to distinguish C. autumnale simply and rapidly from various other crops.

References

Oct 2, 2001·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·T MatsuokaA Hino
Nov 17, 2012·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Atsushi MasayamaMitsuru Shimizu
Mar 7, 2014·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Miou TodaFumiko Kasuga
Apr 8, 2014·Reumatismo·A AghabiklooeiM Mashayekhian
Jan 3, 2015·The American Journal of Medicine·Anastasia SlobodnickSvetlana Krasnokutsky
May 10, 2017·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Atsuko UshiyamaShigeki Hashiguchi
Jul 12, 2017·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Yohei SuganoKazunari Kondo
Jul 12, 2017·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Chie NomuraKeiji Kajimura

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