A rapid and simple method for the measurement of plasma 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 using Sep-Pak C18 cartridges and a single high-performance liquid chromatographic step

Clinica Chimica Acta; International Journal of Clinical Chemistry
H TurnbullH L Makin

Abstract

A simple one step high-performance liquid chromatographic (HPLC) procedure for the analysis of plasma concentrations of 25-hydroxyvitamin D3 (25-OHD3) and 25-hydroxyvitamin D2 (25-OHD2) is described. Plasma (2-4 ml) was extracted with methyl cyanide which was passed through a Sep-Pak C18 cartridge, washed with methanol:water (70:30, v/v) and the 25-OHD fraction eluted with methyl cyanide. After isomerisation to their isotachysterol derivatives, the secosteroids were estimated using a straight-phase HPLC system, monitoring the eluent at 301 nm. Radioactive 25-OHD3, added to plasma at the start of the procedure, was used to correct for losses. Recovery of added 25-OHD3 was quantitative and values obtained using this method were similar to those obtained on the same plasma samples using a mass fragmentographic technique. Normal ranges were similar to those described by other workers and within- (5.8% for 25-OHD3) and between-(8.0% for 25-OHD3) batch reproducibilities were satisfactory.

References

Jul 15, 1979·The Biochemical Journal·R M ShepardH F DeLuca
Nov 3, 1977·The New England Journal of Medicine·L V Avioli, J G Haddad
Dec 1, 1971·The Journal of Clinical Endocrinology and Metabolism·J G Haddad, K J Chyu
Sep 8, 1980·Clinica Chimica Acta; International Journal of Clinical Chemistry·D A SeamarkH L Makin
Nov 6, 1980·Clinica Chimica Acta; International Journal of Clinical Chemistry·C H Shackleton, J O Whitney
Dec 8, 1980·Clinica Chimica Acta; International Journal of Clinical Chemistry·G A TaylorA Holmes
Mar 1, 1980·Clinics in Endocrinology and Metabolism·E B Mawer
Jan 1, 1981·Journal of Steroid Biochemistry·D A SeamarkH L Makin
Jan 1, 1980·Journal of Agricultural and Food Chemistry·K T Koshy, A L VanDerSlik
Aug 1, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·O O GrigoriantsD M Desiderio
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

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Citations

Aug 1, 1992·Calcified Tissue International·R J HeikinheimoS A Rajala
Dec 1, 1995·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·M Ala-HouhalaO Koskimies
Mar 28, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·D J Trafford, H L Makin
Aug 14, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·G S ShephardD Labadarios
Aug 31, 1988·Clinica Chimica Acta; International Journal of Clinical Chemistry·B DeanL C Harrison
Dec 1, 1987·Journal of Steroid Biochemistry·C E PorteousH L Makin
May 1, 1996·Maturitas·R HeikinheimoI Vuori
Dec 1, 1985·Archives of Disease in Childhood·N D MartinH L Makin
Dec 1, 1986·Archives of Disease in Childhood·M Ala-HouhalaJ Visakorpi
Apr 22, 1999·Archives of Disease in Childhood. Fetal and Neonatal Edition·M C BackströmM Mäki
Dec 4, 2003·International Journal of Cancer. Journal International Du Cancer·Susanna MiettinenTimo Ylikomi
Nov 24, 1999·Current Opinion in Clinical Nutrition and Metabolic Care·G S Fell, D Talwar
Dec 6, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yan-Ru LouPentti Tuohimaa
Jun 1, 1984·Scandinavian Journal of Clinical and Laboratory Investigation·I HolmbergM Sturén
Jan 1, 1988·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·M J JongenJ C Netelenbos
Nov 21, 1997·Annals of Clinical Biochemistry·C J Bates
Apr 6, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ali K AlshabrawyPaul H Anderson
Jan 1, 1983·Journal of Pharmaceutical and Biomedical Analysis·J W HonourF L Mitchell
Aug 11, 1989·Journal of Chromatography·D C Turnell, J D Cooper
Jul 29, 1988·Journal of Chromatography·A P De LeenheerR M Bauwens
Sep 13, 1985·Journal of Chromatography·B W Hollis, N E Frank

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