PMID: 16538008Mar 16, 2006Paper

A rapid and simple system of detecting deletions on the Y chromosome related with male infertility using multiplex PCR

The Journal of Medical Investigation : JMI
Mayumi UmenoYutaka Nakahori

Abstract

Around 10% of males with idiopathic azoospermia or oligozoospermia, which are important causes of male infertility, have partial deletions on the long arm of the Y chromosome. To develop a rapid and accurate detection system for screening major Y deletions found in infertile men, we developed a multiplex PCR system that can simultaneously amplify five loci on the Y chromosome, SRY, AMELY, DBY, RBMY, DAZ and one locus on the X chromosome, AMELX. The size of the PCR products was designed to increase gradually from the distal Yp to the distal Yq. Our system could detect deletions of three major candidate regions for the azoospermic factor, AZFa, AZFb and AZFc on the Y chromosome together with sex. The gradual increase in the size of the PCR products was convenient for imaging the location of deletions on the Y chromosome. Moreover, the multiplex PCR system was combined with microchip-based electrophoresis, and the PCR products derived from each locus were separated within 4 min. Our system is useful for screening Y chromosomes bearing the structural anomalies including three major AZF deletions found among azoospermic or oligozoospermic males.

References

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Citations

May 15, 2012·Journal of Assisted Reproduction and Genetics·Dingyang LiRuizhi Liu
Sep 1, 2011·Einstein·Bianca BiancoCaio Parente Barbosa
May 12, 2011·International Braz J Urol : Official Journal of the Brazilian Society of Urology·Fernanda A MafraCaio P Barbosa

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