A rapid and simple test system for the evaluation of the inhibitory activity of retinoids on induced ornithine decarboxylase activity in the hairless rat epidermis

Journal of Pharmacological Methods
M BouclierC N Hensby

Abstract

In mouse skin, antiproliferative agents including retinoids inhibit induction of ornithine decarboxylase activity by a variety of hyperproliferative stimuli. In the hairless rat skin, ornithine decarboxylase activity was induced by ten successive strippings with cellotape and by topical application of 12-O-tetradecanoyl-phorbol-13-acetate. Topical application of all trans-retinoic acid (25 nmol/cm2) immediately after the tape stripping of the skin significantly inhibited the induction of ornithine decarboxylase activity at all time points measured. The inhibition by all trans-retinoic acid of ornithine decarboxylase induced by cellotape stripping was dose dependent as was found to be the case for arotinoid, retinol, Ro-10-1670, motretinid, 13-cis-retinoic acid, etretinate, and vitamin A. Oral administration of all trans-retinoic acid also inhibited the ornithine decarboxylase activity induced by cellotape stripping. We propose the assay of ornithine decarboxylase activity in the hairless rat epidermis after tape stripping for a rapid evaluation of new retinoids.

References

Jul 1, 1977·Journal of Medicinal Chemistry·B A PawsonR Rüegg
Aug 1, 1979·Archives of Dermatology·J J Voorhees
Jun 9, 1978·Biochimica Et Biophysica Acta·H PösöJ Jänne
Mar 1, 1981·Archives of Dermatology·P M Elias, M L Williams
Apr 1, 1982·Journal of the American Academy of Dermatology·R K Boutwell
Sep 1, 1982·Archives of Biochemistry and Biophysics·H Pösö, A E Pegg

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Citations

Jan 1, 1989·Pharmacology & Therapeutics·J G Allen, D P Bloxham

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