A rapid assay to screen for drug-resistant herpes simplex virus

The Journal of Infectious Diseases
P TebasP D Olivo

Abstract

A rapid assay was developed to screen for herpes simplex virus (HSV) isolates that are resistant to acyclovir and other antiviral agents. The assay is a modified plaque reduction assay (PRA) in which the number of plaques seen in the absence of acyclovir was compared with that seen in the presence of a single cutoff concentration of acyclovir (2 microg/mL). This assay utilizes a cell line that expresses beta-galactosidase only after infection with HSV. Since histochemically stained plaques are easily visualized, small plaques can be easily enumerated. This allows the assay to be performed on dilutions of untitered specimens in the small wells of a 24-well plate and allows the results to be read only 2 days after inoculation of the virus. The assay performed well compared with a standard PRA and should be a valuable tool in identifying drug-resistant HSV in a timely manner.

Citations

Apr 26, 2005·Journal of Virological Methods·Andrew LutzAndrew Pekosz
Sep 25, 2002·Journal of Virological Methods·Christelle DanveMichèle Aymard
Sep 10, 1999·Antiviral Research·J J McSharry
May 22, 2003·Dermatologic Clinics·Suneel Chilukuri, Ted Rosen
Jun 9, 2001·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·H J Field
May 25, 2004·Antimicrobial Agents and Chemotherapy·Ruzena StránskáAnton M van Loon
Jan 15, 2003·Clinical Microbiology Reviews·Teresa H BaconDavid Sutton
Aug 23, 2008·Journal of Basic Microbiology·Zhe MaWentao Qiao
Oct 31, 2013·International Journal of Environmental Research and Public Health·Bo YangShujuan Li

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