PMID: 2110585Feb 1, 1990Paper

A rapid bioassay for chemicals that induce pro-oxidant states

Journal of Applied Toxicology : JAT
L M KnobelochJ M Harkin

Abstract

A new bioassay has been developed that allows rapid, sensitive detection of chemicals such as paraquat and adriamycin, which manifest their acute toxicity, mutagenicity or carcinogenicity by inducing a pro-oxidant state in vivo. Submitochondrial particles isolated from bovine myocardium are used to catalyze NADH-dependent enzymatic reduction of these chemicals to free radicals. The highly reactive species generated in this system reduce molecular dioxygen to the superoxide anion radical, which is detected spectrophotometrically using the adrenochrome reaction. The anticancer drug adriamycin, the herbicides paraquat and diquat, the analytical dye sulfonazo III, and the experimental carcinogen 4-nitroquinoline-N-oxide have been used to test the sensitivity of this new method. This assay can be used to screen fresh water samples for the presence of pollutants that can generate oxygen-centered free radicals in vivo, or to test newly synthesized chemicals for this activity, and may therefore be valuable for environmental monitoring and preliminary toxicity evaluation of industrial or pharmaceutical products.

References

Aug 1, 1976·Environmental Health Perspectives·J S BusJ E Gibson
Nov 1, 1990·Archives of Environmental Contamination and Toxicology·L M KnobelochJ M Harkin
Jan 25, 1985·Science·P A Cerutti
Jul 1, 1988·Toxicology and Applied Pharmacology·R J Ruch, J E Klaunig
Mar 1, 1987·Bulletin of Environmental Contamination and Toxicology·G A BlondinJ M Harkin

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Citations

May 1, 1990·Bulletin of Environmental Contamination and Toxicology·L M KnobelochJ M Harkin
Feb 18, 2014·Journal of Applied Toxicology : JAT·Peter Kovacic, Ratnasamy Somanathan
Jan 24, 2002·The Journal of Pharmacology and Experimental Therapeutics·Cuiping Chen, Dhiren R Thakker
Nov 27, 2008·Environmental Science & Technology·Delina Y Lyon, Pedro J J Alvarez
Feb 7, 2007·Life Sciences·Periasamy SrinivasanChennam Srinivasulu Shyamaladevi

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