PMID: 2506087Aug 1, 1989Paper

A rapid, Ca2+-independent, onset of prostaglandin E2 stimulation of the release of luteinizing hormone releasing hormone from copper-treated median eminence explants

Molecular and Cellular Endocrinology
A Barnea, K R Bhasker

Abstract

Prostaglandin E2 (PGE2) is known to stimulate the release of luteinizing hormone releasing hormone (LHRH) from explants of the median eminence area (MEA). We have previously shown that a short preincubation of the MEA with copper markedly amplifies PGE2 stimulation of LHRH release and that the Ca2+-cAMP pathway is involved in this release process. In this study, we defined the kinetics of the onset of PGE2 action and examined the Ca2+ requirement for the onset and manifestation of PGE2 action. MEA explants from immature female rats were incubated with copper (150 microM copper-histidine complex); thereafter, explants were exposed to 10 microM PGE2 for periods of 2-7 min with or without Ca2+ (Ca2+-free buffer containing 1 mM EGTA). A 2 min PGE2 exposure was as effective as a 7 min PGE2 exposure in stimulating LHRH release; the latter was manifested during the period between 2-7 min. When MEA were exposed to PGE2 for 5 min, maximal rate of release was attained within this 5 min period. When MEA were exposed to PGE2 for 2 min and then to forskolin (100 microM) for 5 min, there was no further increase in the rate of LHRH release (although exposure to forskolin alone maximally stimulated LHRH release). In the presence of EGTA (no Ca...Continue Reading

References

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