A Rapid Capillary-Pressure Driven Micro-Channel to Demonstrate Newtonian Fluid Behavior of Zebrafish Blood at High Shear Rates

Scientific Reports
Juhyun LeeTzung K Hsiai

Abstract

Blood viscosity provides the rheological basis to elucidate shear stress underlying developmental cardiac mechanics and physiology. Zebrafish is a high throughput model for developmental biology, forward-genetics, and drug discovery. The micro-scale posed an experimental challenge to measure blood viscosity. To address this challenge, a microfluidic viscometer driven by surface tension was developed to reduce the sample volume required (3μL) for rapid (<2 min) and continuous viscosity measurement. By fitting the power-law fluid model to the travel distance of blood through the micro-channel as a function of time and channel configuration, the experimentally acquired blood viscosity was compared with a vacuum-driven capillary viscometer at high shear rates (>500 s-1), at which the power law exponent (n) of zebrafish blood was nearly 1 behaving as a Newtonian fluid. The measured values of whole blood from the micro-channel (4.17cP) and the vacuum method (4.22cP) at 500 s-1 were closely correlated at 27 °C. A calibration curve was established for viscosity as a function of hematocrits to predict a rise and fall in viscosity during embryonic development. Thus, our rapid capillary pressure-driven micro-channel revealed the Newtonian...Continue Reading

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Citations

Aug 26, 2019·Biomechanics and Modeling in Mechanobiology·Yoke Yin FooChoon Hwai Yap
Aug 24, 2018·JCI Insight·Yichen DingTzung K Hsiai
Feb 23, 2020·Biomacromolecules·Victorio Saez TalensRoxanne E Kieltyka
Oct 27, 2021·Journal of the Royal Society, Interface·Sreyashi ChakrabortyPavlos P Vlachos

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BETA
amputation
rheology
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