A rapid chemiluminescent DNA hybridization assay for the detection of Chlamydia trachomatis

Journal of Bioluminescence and Chemiluminescence
J M ClyneM S Urdea

Abstract

With an estimated 3-4 million new cases per year, human infections from Chlamydia trachomatis are probably the most prevalent sexually transmitted disease (STD) in the United States. Diagnosis of Chlamydia is usually conducted by tissue culture methods. Direct immunofluorescence and ELISA tests have become available, but there remains a need for a test with better specificity and sensitivity. In response to this need, we have developed a rapid DNA hybridization assay using synthetic oligonucleotide probes to detect the presence of the Chlamydia trachomatis specific 7.4 kb plasmid. The assay involves solution phase hybridization of unlabelled probes, rapid capture of the probe-target duplex onto a microtitre dish surface, a new signal amplification technique that employs chemically cross-linked oligonucleotides, and an alkaline phosphatase labelled probe. Signal is obtained by reacting the labelled probe-target complex with an enzyme triggerable dioxetane substrate. Detection of the chemiluminescent output is performed either with a luminometer or by exposure to instant film. All 15 serovars of Chlamydia trachomatis react positively, while organisms known to co-inhabit the human urogenital tract react negatively.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jul 1, 1986·Plasmid·L Palmer, S Falkow
Mar 1, 1986·Diagnostic Microbiology and Infectious Disease·J Schachter
Jun 1, 1988·Journal of Clinical Microbiology·M H LamK F Fairley
May 1, 1987·Journal of Clinical Microbiology·J W SmithR B Jones
Feb 1, 1986·Journal of Clinical Microbiology·L V HowardJ E Herrmann
Jun 1, 1986·European Journal of Clinical Microbiology·E ZyzikR Thomssen
Dec 1, 1985·European Journal of Clinical Microbiology·K H TjiamM F Michel
Feb 1, 1986·Infection and Immunity·T JosephH D Caldwell
Nov 15, 1985·Analytical Biochemistry·J A MatthewsL J Kricka
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Church, W Gilbert
Sep 1, 1984·Journal of Clinical Microbiology·M F JonesJ E Herrmann
Aug 1, 1983·Infection and Immunity·C C KuoJ T Grayston
Jan 1, 1980·Annual Review of Microbiology·J Schachter, H D Caldwell

❮ Previous
Next ❯

Citations

May 1, 1994·Journal of Bioluminescence and Chemiluminescence·H Akhavan-TaftiY Sugioka
Dec 17, 1990·Clinica Chimica Acta; International Journal of Clinical Chemistry·N C Nelson, D L Kacian
Oct 31, 2008·Talanta·C DodeigneR Lejeune
Nov 1, 1990·Analytical Chemistry·S Beck, H Köster
Jan 1, 1989·Journal of Clinical Laboratory Analysis·I Bronstein, L J Kricka
Apr 25, 2003·Journal of Neurophysiology·Laurel A GravesTed Abel
Jun 24, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·K A JhaveriL A Toth
Oct 1, 1992·Clinical Microbiology Reviews·M J Wolcott

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.