A rapid confirmatory method for analyzing tetracycline antibiotics in bovine, swine, and poultry muscle tissues: matrix solid-phase dispersion with heated water as extractant followed by liquid chromatography-tandem mass spectrometry

Journal of Agricultural and Food Chemistry
Sara BogialliGabriella Rizzuti

Abstract

A rapid, specific, and sensitive procedure for determining four widely used tetracycline antibiotics and three related epimers in bovine, swine, and poultry muscle tissues is presented. The method is based on the matrix solid-phase dispersion technique with heated water as the extractant followed by liquid chromatography (LC)-tandem mass spectrometry (MS) equipped with an electrospray ion source. Target compounds were extracted from tissues with 5 mL of water heated at 70 degrees C. After acidification and filtration, 100 microL of the aqueous extract was injected in the LC column. MS data acquisition was performed in the multireaction monitoring mode, selecting two precursor ion to product ion transitions for each target compound. Heated water appeared to be an excellent extractant, since the absolute recovery data ranged between 70 and 78%. The accuracy of the method was determined at three spike levels, using minocycline as a surrogate analyte, in any different kind of muscle tissues considered and varied between 88 and 109% with relative standard deviations ranging between 3 and 11%. Limits of quantification were estimated to range between 1 (chlortetracycline) and 9 ng/g (4-epioxytetracycline), based on a signal-to-noise r...Continue Reading

References

Jul 28, 1989·Journal of Chromatography·S A BarkerC R Short
Aug 6, 1998·Journal of Chromatography. a·R W Fedeniuk, P J Shand
Jan 23, 1999·Journal of Chromatography. a·Y LiangR B Bates
Jul 15, 2000·Journal of Chromatography. a·H OkaH Matsumoto
Aug 15, 2000·Journal of Chromatography. a·S A Barker

❮ Previous
Next ❯

Citations

Jan 17, 2008·Analytical and Bioanalytical Chemistry·Dimitrios J Fletouris, Elias P Papapanagiotou
Feb 8, 2008·Analytical and Bioanalytical Chemistry·M García-LópezI Rodríguez
Apr 25, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Anna GajdaGrzegorz Tomczyk
Oct 13, 2011·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·M S BittencourtR Hoff
Mar 18, 2008·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Maki KandaToshihiro Nagayama
Jul 18, 2009·Analytical and Bioanalytical Chemistry·Sara Bogialli, Antonio Di Corcia
Feb 2, 2010·Journal of Chromatography. a·Anna Laura CapriottiAldo Laganà
Sep 29, 2009·Journal of Chromatography. a·Brian KinsellaMartin Danaher
Mar 11, 2009·Journal of Chromatography. a·H F De BrabanderS Croubels
Feb 24, 2009·Journal of Chromatography. a·J M MirandaC A Galán-Vidal
Apr 19, 2011·Mass Spectrometry Reviews·Helga TrufelliAchille Cappiello
Nov 28, 2006·Journal of Biochemical and Biophysical Methods·Sara Bogialli, Antonio Di Corcia
Nov 17, 2006·Journal of Biochemical and Biophysical Methods·Steven A Barker
Dec 1, 2006·Journal of Mass Spectrometry : JMS
Feb 28, 2015·The Journal of Dairy Research·Melisa TuminiRafael Lisandro Althaus

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.