PMID: 6161111Mar 1, 1980Paper

A rapid cytofluorometric method for quantitative DNA determination on fixed smears

The Histochemical Journal
G MazziniA Riccardi

Abstract

A study has been made of the possibility of using propidium iodide (PI) for the cytofluorometric determination of DNA in fixed smears. A preliminary spectrofluorometric study made it possible to define the optimal conditions for the excitation wavelength and the intensity of the emitted fluorescence. The stoichiometry and specificity of the DNA-PI bond were studied in different materials and for different fixation procedures. In order to check whether RNA might interfere, it was extracted enzymatically from test preparations selectively. The data from such treated sections were not significantly different from those obtained by means of the fluorescent Feulgen reaction (Pararosaniline-SO2) on the same material. On the other hand, some of the advantages of the PI method are important: (a) the time required for making ready and staining the preparations is very short, and in any case is considerably shorter than the Feulgen method; (b) the high quantum yield of the DNA-PI complex induces very high fluorescence intensities, which can, therefore, be easily measured, even with low sensitivity instruments; (c) the spectral conditions are particularly favourable for excluding the inner filter effect from the measurement; (d) the photo...Continue Reading

References

Feb 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E Johannisson, B Thorell
Dec 1, 1977·Analytical Biochemistry·K J Andersen, D W Skagen
Feb 1, 1975·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·U TorelliR Cadossi
Jun 21, 1974·Science·H A Crissman, R A Tobey
Sep 1, 1974·The Histochemical Journal·G PrennaG Mazzini
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Jan 1, 1968·Histochemie. Histochemistry. Histochimie·N Böhm, E Sprenger
Mar 14, 1969·Science·M A Van DillaJ R Coulter

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Citations

Aug 1, 1995·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Y NakaiS Kohno
Jun 1, 2005·Journal of Biomedical Materials Research. Part a·B BaharlooD M Brunette

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