A rapid diagnostic multiplex PCR approach for xenomonitoring of human and animal schistosomiasis in a 'One Health' context

Transactions of the Royal Society of Tropical Medicine and Hygiene
Ruben ScholsT Huyse

Abstract

Studying the epidemiology of schistosomiasis-the most prevalent gastropod-borne human disease and an economic burden for the livestock industry-relies on adequate monitoring tools. Here we describe a molecular assay for detecting human and animal African schistosome species in their planorbid gastropod host (xenomonitoring) using a two-step approach. First, schistosome infections are detected and discriminated from other trematode infections using a multiplex polymerase chain reaction (PCR) that includes a trematode-specific marker (in 18S rDNA), a Schistosoma genus-specific marker (in internal transcribed spacer 2 [ITS2]) and a general gastropod marker (in 18S rDNA) as an internal control. Upon Schistosoma sp. detection, a second multiplex PCR is performed to discriminate among Schistosoma haematobium, Schistosoma mansoni, Schistosoma mattheei and Schistosoma bovis/Schistosoma curassoni/Schistosoma guineensis using markers of differential lengths in the cytochrome c oxidase subunit 1 (COX1) gene. The specificity of these assays was validated with adult worms, naturally infected gastropods and human urine and stool samples. Sensitivity was tested on experimentally infected snail specimens that were sacrificed 10 and 40 days pos...Continue Reading

References

Jan 9, 1998·International Journal for Parasitology·D T LittlewoodK A Clough
Mar 5, 2003·Proceedings. Biological Sciences·Paul D N HebertJeremy R deWaard
Apr 3, 2003·Genome Génome / Conseil National De Recherches Canada·Cheryl D ProkopowichTeresa J Crease
Jan 30, 2004·Letters in Applied Microbiology·J HoorfarP Fach
Oct 19, 2005·Advances in Parasitology·Matthew J Nolan, Thomas H Cribb
Sep 26, 2006·Lancet·Bruno GryseelsLuc Kestens
May 25, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·F Van den BroeckT Huyse
Dec 5, 2012·The American Journal of Tropical Medicine and Hygiene·Joseph HamburgerCharles H King
May 2, 2014·Parasitology Research·J RouttuF Ben-Ami
Aug 2, 2017·Trends in Parasitology·Nils PilotteSteven A Williams
Oct 19, 2017·Transactions of the Royal Society of Tropical Medicine and Hygiene·Charlotte M GowerJoanne P Webster
Oct 3, 2018·Tropical Medicine and Infectious Disease·Hong YouDonald P McManus
May 18, 2019·The Science of the Total Environment·Hans CarolusTine Huyse

❮ Previous
Next ❯

Citations

Sep 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tom PennanceBonnie Lee Webster
Feb 5, 2021·Journal of Clinical Medicine·Juan García-Bernalt DiegoAntonio Muro
Jul 1, 2020·The Lancet Infectious Diseases·Abena S AmoahGovert J van Dam
Jun 3, 2021·International Journal of Environmental Research and Public Health·Onyekachi Esther NwokoMoses John Chimbari
Jul 3, 2021·Tropical Medicine and Infectious Disease·Oyime Poise AulaCatherine A Gordon
Oct 5, 2021·PLoS Neglected Tropical Diseases·Ebrima JoofChristopher M Wade

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.