A rapid, direct assay to measure degranulation of bovine neutrophil primary granules

Veterinary Immunology and Immunopathology
M J Quade, J A Roth

Abstract

A direct, rapid, quantitative colorimetric assay to determine neutrophil primary granule degranulation was adapted for use with bovine neutrophils. The assay measures the exocytosis of myeloperoxidase (MPO) using 3,3',5,5'-tetramethylbenzidine as a substrate. The assay was validated by evaluating the effects of various stimulants and inhibitors of degranulation, the kinetics of primary granule exocytosis, and comparing the total myeloperoxidase content of neutrophils obtained from calves and adults. The results demonstrate that the assay is capable of detecting important differences that may occur in degranulation of bovine neutrophil primary granules and in total neutrophil myeloperoxidase content.

References

Mar 1, 1978·Proceedings of the Society for Experimental Biology and Medicine·R GennaroD Romeo
Jul 1, 1991·Veterinary Immunology and Immunopathology·G B Brown, J A Roth
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Apr 1, 1981·Veterinary Immunology and Immunopathology·J A Roth, M L Kaeberle

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Citations

Mar 30, 2010·Fish Physiology and Biochemistry·Christopher Marlowe A CaipangViswanath Kiron
Dec 31, 2010·Fish Physiology and Biochemistry·Christopher Marlowe A CaipangViswanath Kiron
Jun 25, 2005·Developmental and Comparative Immunology·Irene SalinasJosé Meseguer
May 10, 2005·Life Sciences·Xue-Ling LiaoMan-Ping Wu
Oct 18, 2002·Veterinary Immunology and Immunopathology·Jeanne M du ManoirJeff L Caswell
Jan 15, 2014·Fish & Shellfish Immunology·Francisco A GuardiolaMaría A Esteban
Nov 12, 2013·Fish Physiology and Biochemistry·Nadjoua ChebaaniAlberto Cuesta
May 24, 2014·Fish & Shellfish Immunology·Yulema ValeroElena Chaves-Pozo
Apr 22, 2003·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Alejandro RodríguezJosé Meseguer
Jul 20, 2011·Fish & Shellfish Immunology·Martha Reyes-BecerrilMaría Ángeles Esteban
Jul 26, 2011·Fish & Shellfish Immunology·Ramasamy HarikrishnanMoon-Soo Heo
Dec 21, 2010·Fish & Shellfish Immunology·F A GuardiolaM A Esteban
Nov 10, 2015·Fish & Shellfish Immunology·Veluchamy RajeswariRajaiah Shenbagarathai
Jan 27, 2009·Comparative Immunology, Microbiology and Infectious Diseases·Alba Miranda-RiberaFabrizio Ceciliani
Dec 9, 2008·Fish & Shellfish Immunology·Rebeca CerezuelaMa Angeles Esteban

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