A rapid fluorescence based method for the quantitative analysis of cell culture media photo-degradation

Analytica Chimica Acta
Amandine CalvetAlan G Ryder

Abstract

Cell culture media are very complex chemical mixtures that are one of the most important aspects in biopharmaceutical manufacturing. The complex composition of many media leads to materials that are inherently unstable and of particular concern, is media photo-damage which can adversely affect cell culture performance. This can be significant particularly with small scale transparent bioreactors and media containers are used for process development or research. Chromatographic and/or mass spectrometry based analyses are often time-consuming and expensive for routine high-throughput media analysis particularly during scale up or development processes. Fluorescence excitation-emission matrix (EEM) spectroscopy combined with multi-way chemometrics is a robust methodology applicable for the analysis of raw materials, media, and bioprocess broths. Here we demonstrate how EEM spectroscopy was used for the rapid, quantitative analysis of media degradation caused by ambient visible light exposure. The primary degradation pathways involve riboflavin (leading to the formation of lumichrome, LmC) which also causes photo-sensitised degradation of tryptophan, which was validated using high pressure liquid chromatography (HPLC) measurements....Continue Reading

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Sep 4, 2012·Journal of Pharmaceutical and Biomedical Analysis·Amandine CalvetAlan G Ryder

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Citations

Apr 8, 2014·Analytica Chimica Acta·Radu Constantin GrozaAlan G Ryder
Sep 1, 2015·Analytica Chimica Acta·Radu Constantin GrozaAlan G Ryder
May 24, 2017·Applied Spectroscopy·Kevin Buckley, Alan G Ryder
Aug 14, 2018·Methods and Applications in Fluorescence·Dzmitry MelnikauAlan G Ryder
Sep 25, 2017·Biotechnology Letters·Nicola McGillicuddyJonathan Bones

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