PMID: 9438561Jan 23, 1998Paper

A rapid gas chromatographic assay for determining oxyradical scavenging capacity of antioxidants and biological fluids

Free Radical Biology & Medicine
G W WinstonK A Blanchard

Abstract

Herein, we report a new, rapid,and reliable method for measuring the protective antioxidant potential of pure antioxidant solutions or biological tissues. Peroxyl radicals generated by thermal homolysis of 2,2'-azobis-amidinopropane (ABAP) cause the oxidation of alpha-keto-gamma-methiolbutyric acid (KMBA) to ethylene; ethylene formation is monitored by gas chromatographic analysis of head space from the reaction vessel. The partial inhibition of ethylene formation in the presence of antioxidants that compete with KMBA for oxyradicals is the basis of the Total Oxyradical Scavenging Capacity Assay (TOSCA). The assay is shown to be reliable for quantifying ROS scavenging potential. The quantifiable parameters are consistent with the relative order of those predicted by the fluorescence- and oxygen electrode-based assays reported in the literature. Antioxidants competing for peroxyl radicals influenced the rate of KMBA oxidation in different ways, but the calculation of TOSC was not affected by such variations. Responses were linear over a wide range of sample concentrations and the TOSC values of classical soluble antioxidants showed the following relative order: Trolox > uric acid > ascorbic acid > GSH. The KMBA method was reliab...Continue Reading

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Citations

Mar 13, 2009·Archives of Pharmacal Research·Do Young KwonYoung Chul Kim
Jul 8, 2010·Archives of Pharmacal Research·Sun Ju KimYoung Chul Kim
Mar 20, 2013·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Roberta Oliveira LobatoJuliane Ventura-Lima
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Sep 12, 2015·Environmental Science and Pollution Research International·Lucas CordeiroJuliane Ventura-Lima

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