A rapid GC-MS determination of gamma-hydroxybutyrate in saliva

Journal of Analytical Toxicology
Giorgia De Paoli, Suzanne Bell

Abstract

GHB and its related compounds have been known for years in forensic toxicology because of their illicit use in drug-facilitated sexual assault, and to a lesser extent, as party drugs. A sensitive and specific method for the identification and quantification of gamma-hydroxybutyric acid (GHB) in saliva has been developed using gas chromatography-mass spectrometry with selective ion monitoring mode. One microliter of synthetic saliva was spiked with 1.0 microL of GHB-d(6) as the internal standard and 1.0 microL of 1,7-heptanediol as a surrogate spike to all samples. After a silyl-derivatization, the sample was injected at a split ratio of 10:1. The following ions were monitoring: 233 and 234 for GHB; 239, 240, and 241 for GHB-d(6); and 55, 73, and 97 for 1,7-heptanediol. The limit of quantitation was determined to be 0.5 mg/L with a linear dynamic range of 0.5-50.0 mg/L. Quality control samples (5.0, 20.0, and 30.0 mg/L) were prepared for the evaluation of precision. Analytical precision measured by coefficients of variation ranged from 2.1% to 12.50% in both intraday and day-to-day experiments. Surrogate recovery from saliva samples fell in the range of 94.6% to 100% with an average of 98.37% and a corresponding percent relative...Continue Reading

Citations

Feb 7, 2014·Analytical and Bioanalytical Chemistry·Ann-Sofie M E IngelsChristophe P Stove
Sep 15, 2009·Environmental Science & Technology·Michael KovochichMark R Wiesner
Sep 4, 2012·Clinics in Laboratory Medicine·Donald L Frederick
Nov 10, 2010·Biomedical Chromatography : BMC·Stacy D Brown, Tyler C Melton
Jul 19, 2016·Journal of Pharmaceutical and Biomedical Analysis·Laura Mercolini, Michele Protti
Nov 23, 2011·Therapeutic Drug Monitoring·Lara HasanAndré Scholer
May 9, 2009·Analytical Chemistry·T A BrettellJ R Almirall

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