A Rapid Growth-Independent Antibiotic Resistance Detection Test by SYBR Green/Propidium Iodide Viability Assay

Frontiers in Medicine
Jie FengYing Zhang

Abstract

Antibiotic-resistant bacteria have caused huge concerns and demand innovative approaches for their prompt detection. Current antimicrobial susceptibility tests (AST) rely on the growth of the organisms which takes 1-2 days for fast-growing organisms and several weeks for slow growing organisms. Here, we show for the first time the utility of the SYBR Green I/propidium iodide (PI) viability assay for rapidly identifying antibiotic resistance in less than 30 min for major, antibiotic-resistant, fast-growing bacteria, such as Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Acinetobacter baumannii for bactericidal and bacteriostatic agents and in 16 h for extremely rapid detection of drug resistance for isoniazid and pyrazinamide in slow-growing Mycobacterium tuberculosis. The SYBR Green I/PI assay generated rapid and robust results in concordance with traditional AST methods. This novel growth-independent methodology changes the concept of the current growth-based AST and may revolutionize current drug susceptibility testing for all cells of prokaryotic and eukaryotic origin and, subject to further clinical validation, may play a major role in saving lives and improving patient outcomes.

References

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Citations

Sep 7, 2018·Respirology : Official Journal of the Asian Pacific Society of Respirology·Paolo MiottoWing Cheong Yam
Jul 20, 2019·Animal Science Journal = Nihon Chikusan Gakkaihō·Xuekai TianTaiyong Yu
Oct 22, 2019·Frontiers in Microbiology·Rebecca YeeYing Zhang
Apr 27, 2021·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Cigdem SahinNazime Mercan Dogan

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Methods Mentioned

BETA
flow cytometry
fluorescence microscopy
Assay
Fluorescence

Software Mentioned

Excel
X Analyzer
GraphPad Prism
BZ
Image Pro - Plus

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