A Rapid Immunochromatography Test Based on Hcp1 Is a Potential Point-of-Care Test for Serological Diagnosis of Melioidosis

Journal of Clinical Microbiology
Phornpun PhokraiNarisara Chantratita

Abstract

Melioidosis is a fatal infectious disease caused by the environmental bacterium Burkholderia pseudomallei It is highly endemic in Asia and northern Australia but neglected in many other tropical countries. Melioidosis patients have a wide range of clinical manifestations, and definitive diagnosis requires bacterial culture, which can be time-consuming. A reliable rapid serological tool is greatly needed for disease surveillance and diagnosis. We previously demonstrated by enzyme-linked immunosorbent assay (ELISA) that a hemolysin-coregulated protein (Hcp1) is a promising target for serodiagnosis of melioidosis. In this study, we developed a rapid immunochromatography test (ICT) using Hcp1 as the target antigen (Hcp1-ICT). We evaluated this test for specific antibody detection using serum samples obtained from 4 groups of human subjects, including the following: (i) 487 culture-confirmed melioidosis patients from four hospitals in northeast Thailand; (ii) 202 healthy donors from northeast Thailand; (iii) 90 U.S. healthy donors; and (iv) 207 patients infected with other organisms. Compared to culture results as a gold standard, the sensitivity of ICT for all hospitals was 88.3%. The specificities for Thai donors and U.S. donors w...Continue Reading

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Citations

Mar 13, 2020·Clinical Microbiology Reviews·I GassiepR Norton
Oct 23, 2019·Transactions of the Royal Society of Tropical Medicine and Hygiene·Maria Chiara RizziDavid A B Dance
Aug 26, 2020·Travel Medicine and Infectious Disease·Sophie Alexandra BaronGrégory Dubourg
Nov 3, 2021·PLoS Neglected Tropical Diseases·Premjit AmornchaiDirek Limmathurotsakul

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Methods Mentioned

BETA
PCR
enzyme-linked immunosorbent assays
ELISA
Assay

Software Mentioned

BLASTP
Stata

Related Concepts