PMID: 7522589Jul 1, 1994Paper

A rapid immunostaining method for frozen sections

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
M ChilosiF Menestrina

Abstract

A simple and rapid one-step method for demonstrating immunohistochemical markers (leukocyte common antigen, cytokeratin, etc.) is described, which can help define the nature of poorly differentiated neoplasms for diagnosis using frozen section. Microwave irradiation was used to speed immunohistochemical analysis using "Enhanced Polymer One-step Staining" (EPOS) reagents on cryostat sections from a variety of pathologic samples. Reproducible results were obtained using EPOS reagents for leukocyte common antigen and cytokeratin. The overall procedure takes less than 10 min and can be completed during surgery.

References

Jan 1, 1992·The American Journal of Surgical Pathology·O Dworak, C Wittekind
Nov 1, 1990·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M ChilosiF Menestrina
Feb 1, 1986·The Journal of Pathology·A S Leong, J Milios
Jan 1, 1989·American Journal of Clinical Pathology·S S Tabibzadeh, K D Shah
May 1, 1985·American Journal of Clinical Pathology·R V LloydB S Wilson
Nov 24, 1983·The New England Journal of Medicine·R A WarnkeD Y Mason

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Citations

Nov 25, 2000·Breast Cancer : the Journal of the Japanese Breast Cancer Society·M NoguchiN Tonami
Oct 24, 2000·Biomolecular Engineering·S IwamotoM Bothwell
May 29, 2004·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Tokimasa KumadaYasuo Takano
Jun 27, 2003·Cornea·Huaqing GongTsugio Amemiya
Mar 1, 2003·Applied Immunohistochemistry & Molecular Morphology : AIMM·Robyn A BeachCynthia Cohen
Mar 17, 2006·Applied Immunohistochemistry & Molecular Morphology : AIMM·Elizabeth I JohnstonCynthia Cohen
Nov 24, 1999·Journal of Clinical Pathology·T RichterM Werner
Dec 17, 2014·Laboratory Investigation; a Journal of Technical Methods and Pathology·Daniel E Carvajal-HausdorfDavid L Rimm
Apr 17, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·U KämmererP Ruck
Mar 3, 2004·Journal of Surgical Oncology·Giuseppe VialeGiovanni Mazzarol
Jul 21, 2015·The American Journal of Dermatopathology·Amanda F MarschDirk M Elston
Dec 30, 1998·Annals of Surgical Oncology·R R Turner, A E Giuliano
Jan 1, 1997·Acta Oto-laryngologica. Supplementum·T Rott, D Velkavrh
Jun 18, 2021·Journal of Reproductive Immunology·Cihan Deniz KeleşSema Kurnaz

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