A rapid in vivo zebrafish model to elucidate oxidative stress-mediated PCB126-induced apoptosis and developmental toxicity

Free Radical Biology & Medicine
Han LiuWeimin Wang

Abstract

Dioxin-like 3,3',4,4',5-pentachlorobiphenyl (PCB126) is one of the most potent and widespread environmental pollutants. Although PCB126-induced toxicity is related to the aryl hydrocarbon receptor pathway, there is still no study that has constructed an in vivo visual model to clarify the role of the Nrf2/ARE signaling pathway in the oxidative stress mechanism of PCB126-induced toxicity. In the present study, an in vivo zebrafish model of nrf2a fused to enhanced green fluorescent protein (nrf2a-eGFP) was constructed. The zebrafish embryos microinjected with nrf2a-eGFP (72h postfertilization) were exposed to various concentrations of PCB126 (0, 25, 50, 100, 200μg/L) or 30mMN-acetylcysteine (NAC)+200μg/L PCB126. After 72h exposure, PCB126 significantly increased the malformation rates and induced eGFP expression in a dose-dependent manner in several zebrafish tissue types. The distribution of eGFP fluorescence coincided with developmental deformity sites. NAC pretreatment effectively counteracted PCB126-induced developmental toxicity including heart rate, pericardial edema, and body length. The highest PCB126 dose, 200μg/L, produced marked apoptosis in the eye, gill, and trunk detected by the terminal deoxynucleotidyl transferase...Continue Reading

References

Jan 14, 1999·Genes & Development·J MimuraY Fujii-Kuriyama
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·Y Z GuC A Bradfield
Jan 10, 2002·Nature·Karim S EchtayMartin D Brand
Jan 11, 2002·Developmental Biology·L K Cole, L S Ross
Jan 29, 2002·Toxicological Sciences : an Official Journal of the Society of Toxicology·Hiroki TeraokaTakeo Hiraga
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 5, 2004·Environmental Science & Technology·Richard H M M SchreursBart Van der Burg
Apr 28, 2004·Free Radical Biology & Medicine·Anil K Jaiswal
Sep 21, 2004·Annual Review of Plant Biology·Klaus Apel, Heribert Hirt
Dec 16, 2004·Environmental Science & Technology·Paulo S M Carvalho, Donald E Tillitt
Jan 7, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Dagmara S AntkiewiczWarren Heideman
Feb 18, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Heather M Handley-GoldstoneJohn J Stegeman
May 12, 2005·Environmental Science & Technology·Kanta KurauchiMasato Kinoshita
Jan 24, 2006·The Biochemical Journal·Valeska VollrathJose Chianale
Dec 14, 2007·Environmental Science & Technology·Jerome CachotRichard Winn
Dec 14, 2007·Seminars in Cell & Developmental Biology·Natalie LassenVasilis Vasiliou
Jan 1, 2008·Mutation Research·William O Osburn, Thomas W Kensler
May 13, 2008·Drug Discovery Today·Patricia McGrath, Chun-Qi Li
Feb 3, 2009·The Journal of Biological Chemistry·Truyen NguyenCecil B Pickett
Feb 24, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Alicia R Timme-LaragyRichard T Di Giulio
Jun 6, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Matthew J JennyMark E Hahn
Feb 4, 2010·Toxicology and Applied Pharmacology·Curtis D Klaassen, Scott A Reisman
Mar 5, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Xiongjie Shi, Bingsheng Zhou
Aug 19, 2011·Toxicology and Applied Pharmacology·Hong LuCurtis D Klaassen
Dec 17, 2011·The Journal of Biological Chemistry·Alicia R Timme-LaragyMark E Hahn
Jan 25, 2012·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Lindsey V T Garner, Richard T Di Giulio
Jun 9, 2012·Toxicology and Applied Pharmacology·Kai C WuCurtis D Klaassen

❮ Previous
Next ❯

Citations

Nov 12, 2016·Chemosphere·Andrey MassarskyRichard T Di Giulio
Jan 17, 2020·Environmental Science and Pollution Research International·Luigi ViganòPierluisa Dellavedova
Sep 4, 2015·Molecular and Cellular Biochemistry·Ana-Maria Enciu, Laurentiu M Popescu
May 26, 2017·Environmental Monitoring and Assessment·Yunpeng YuRavi Gooneratne
Aug 19, 2018·Scientific Reports·Sebastian Lungu-MiteaJohan Lundqvist
Jun 29, 2021·Environmental Pollution·Jason A CoralMaria Bondesson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis