A rapid interference between glucocorticoids and cAMP-activated signalling in hypothalamic neurones prevents binding of phosphorylated cAMP response element binding protein and glucocorticoid receptor at the CRE-Like and composite GRE sites of thyrotrophin-releasing hormone gene promoter

Journal of Neuroendocrinology
M Y Díaz-GallardoP Joseph-Bravo

Abstract

Glucocorticoids or cAMP increase, within minutes, thyrotrophin-releasing hormone (TRH) transcription in hypothalamic primary cultures, although this effect is prevented if cells are simultaneously incubated with both drugs. Rat TRH promoter contains a CRE site at -101/-94 bp and a composite GRE element (cGRE) at -218/-197 bp. Nuclear extracts of hypothalamic cells incubated with 8Br-cAMP or dexamethasone, and not their combination, bind to oligonucleotides containing the CRE or cGRE sequences. Adjacent to CRE are Sp/Krüppel response elements, and flanking the GRE half site, two AP1 binding sites. The present study aimed to identify the hypothalamic transcription factors that bind to these sites. We verified that the effects of glucocorticoid were not mimicked by corticosterone-bovine serum albumin. Footprinting and chromatin immunoprecipitation (ChIP) assays were used to examine the interaction of cAMP- and glucocorticoid-mediated regulation of TRH transcription at the CRE and cGRE regions of the TRH promoter. Nuclear extracts from hypothalamic cells incubated for 1 h with cAMP or glucocorticoids protected CRE. The GRE half site was recognised by nuclear proteins from cells stimulated with glucocorticoids and, for the adjacent ...Continue Reading

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Citations

Dec 27, 2012·Molecular and Cellular Endocrinology·Dariusz RatmanKarolien De Bosscher
Jul 28, 2011·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Stéphanie Decherf, Barbara A Demeneix
Dec 25, 2010·Molecular and Cellular Endocrinology·Carlos Pérez-MonterLeonor Pérez-Martínez
Jan 27, 2011·Biochimica Et Biophysica Acta·Antonieta Cote-VélezPatricia Joseph-Bravo
Jul 16, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Mi-Sook LeeSong Her
Jan 8, 2015·The Journal of Endocrinology·Patricia Joseph-BravoJean-Louis Charli
Aug 16, 2016·Reviews in Endocrine & Metabolic Disorders·Patricia Joseph-BravoJean-Louis Charli
Mar 25, 2017·Molecular Medicine Reports·Nae TakizawaHisao Yamada

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