PMID: 2498572Apr 17, 1989Paper

A rapid laser immunonephelometric assay for serum amyloid A (SAA) and its application to the diagnosis of kidney allograft rejection

Klinische Wochenschrift
G HockeA Steinmetz

Abstract

We set up a laser nephelometric assay for the quantitation of serum amyloid A (SAA) in human plasma. Therefore monospecific antibodies were raised in sheep and used in parallel to measure SAA concentrations by nephelometry and also by radial immunodiffusion, an assay usually applied for determination of SAA. The nephelometric method is precise, simple and unlike radial immunodiffusion results are obtained within an hour. The antigen concentrations determined both by laser nephelometry and radial immunodiffusion correlated highly (r = 0.98). As plasma SAA concentrations were reported to be a possible marker of kidney allograft rejection, the assay was applied to measure SAA concentrations in patients after kidney transplantation. Data were compared with clinical and other biochemical parameters. The period after kidney transplantation is reported for two cases, where SAA plasma concentrations were helpful in diagnosing allograft rejection. The rapid availability of the SAA plasma concentrations by nephelometry makes them a possible additional tool to decide quickly upon antirejection therapy.

References

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Citations

Dec 1, 1994·The Clinical Investigator·G FeussnerR Ziegler
Nov 28, 1989·Biochimica Et Biophysica Acta·A SteinmetzJ C Fruchart
Sep 1, 1993·Atherosclerosis·E MalleJ G Raynes
Nov 22, 1991·Journal of Immunological Methods·T L McDonaldJ W Smith
Oct 6, 1998·Clinica Chimica Acta; International Journal of Clinical Chemistry·T F MüllerM M Müller
Jan 1, 1996·Transplant International : Official Journal of the European Society for Organ Transplantation·T F MüllerH Lange
Jan 1, 1997·Transplant International : Official Journal of the European Society for Organ Transplantation·T F MüllerH Lange
Feb 1, 1993·Archives of Disease in Childhood·H MiwataT Morishima
Jun 16, 1999·Clinical Chemistry and Laboratory Medicine : CCLM·T Yamada
Dec 24, 2004·Srpski arhiv za celokupno lekarstvo·Dijana B Jovanović
May 9, 2003·The Journal of Veterinary Medical Science·Kimikazu SasakiTakafumi Onishi
Jul 6, 2000·Scandinavian Journal of Infectious Diseases·C PizziniV Fanos
Mar 1, 1995·Annals of Clinical Biochemistry·M T CaslS Kranjcević
Dec 31, 1991·Biochemical and Biophysical Research Communications·B Kluve-BeckermanA Steinmetz

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