A rapid LC-MS/MS quantification of peramivir using a simple and inexpensive sample precipitation: application to PK

Bioanalysis
Zhan-Zhang WangYu-Guan Wen

Abstract

Peramivir is a newly approved selective neuraminidase inhibitor designed to inhibit influenza virus infection. We report a robust and sensitive method utilizing simple precipitation extraction with LC-MS/MS for the high-throughput quantification. Addition of 0.06 M of ammonium formate and 0.1% formic acid in mobile phase could help reduce the matrix effect. This method uses 100 µl of plasma and covers a linear concentration range from 5 to 10,000 ng/ml. Other validation parameters are also evaluated and meet regulatory expectations by US FDA guidelines. The developed HPLC-MS/MS method has been successfully applied to support a clinical pharmacokinetic study. The strategy presented here can be applied elsewhere and may be useful for other amphiphilic drugs.

References

Oct 11, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·M C García
Mar 17, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ying LiZhenqing Zhang
Jul 16, 2011·Bioanalysis·Zhengqi YeChristopher L Brummel
Jul 20, 2011·Obstetrics and Gynecology·Patrick G ClayMarypeace McRae
Oct 4, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·G P HanssonC J Sennbro
Jul 24, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yu-Guan WenDe-Wei Shang
Nov 28, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xin LiZhenqing Zhang
Dec 3, 2013·Journal of Biomolecular Techniques : JBT·Darryl JohnsonRon Orlando
Feb 15, 2014·World Journal of Clinical Cases·Elena LionettiMario La Rosa
May 20, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xiao-Jia NiYu-Guan Wen

Related Concepts

Peramivir
Liquid Chromatography
Cyclopentenes
Guanidines
Chemical Precipitation
Analytic Sample Preparation Methods
Tandem Mass Spectrometry
Ammonium
Amphiphilic Agents
Extracellular Matrix

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