A rapid LC-MS/MS quantification of peramivir using a simple and inexpensive sample precipitation: application to PK

Zhan-Zhang WangYu-Guan Wen


Peramivir is a newly approved selective neuraminidase inhibitor designed to inhibit influenza virus infection. We report a robust and sensitive method utilizing simple precipitation extraction with LC-MS/MS for the high-throughput quantification. Addition of 0.06 M of ammonium formate and 0.1% formic acid in mobile phase could help reduce the matrix effect. This method uses 100 µl of plasma and covers a linear concentration range from 5 to 10,000 ng/ml. Other validation parameters are also evaluated and meet regulatory expectations by US FDA guidelines. The developed HPLC-MS/MS method has been successfully applied to support a clinical pharmacokinetic study. The strategy presented here can be applied elsewhere and may be useful for other amphiphilic drugs.


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Jul 24, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yu-Guan WenDe-Wei Shang
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Dec 3, 2013·Journal of Biomolecular Techniques : JBT·Darryl JohnsonRon Orlando
Feb 15, 2014·World Journal of Clinical Cases·Elena LionettiMario La Rosa
May 20, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xiao-Jia NiYu-Guan Wen

Related Concepts

Liquid Chromatography
Chemical Precipitation
Analytic Sample Preparation Methods
Tandem Mass Spectrometry
Amphiphilic Agents
Extracellular Matrix

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