A rapid magnetic particle-based enzyme immunoassay for human cytomegalovirus glycoprotein B quantification

Journal of Pharmaceutical and Biomedical Analysis
F PiresJuan R Castillo

Abstract

Human cytomegalovirus (HCMV) is a herpes virus that can cause severe infections. Still, the available methods for its diagnostic have the main disadvantage of requiring long time to be performed. In this work, a simple magnetic particle-based enzyme immunoassay (mpEIA) for the quantification of glycoprotein B of Human cytomegalovirus (gB-HCMV) in urine samples is proposed. The immunosensor scheme is based on the analyte protein gB-HCMV sandwiched between a primary monoclonal antibody, (MBs-PrG-mAb1), and a secondary anti-gB-HCMV antibody labelled with Horseradish peroxidase (Ab2-HRP) to allow spectrophotometric detection. The mpEIA analytical performance was tested in urine samples, showing a linear dependence between gB-HCMV concentration and the absorbance signal at 450 nm in a range of concentrations from 90 to 700 pg mL-1. The calculated detection limits for gB-HCMV were 90 ± 2 pg mL-1 and the RSD was about 6.7% in urine samples. The immunosensor showed good selectivity against other viruses from Herpesviridae family, namely varicella zoster and Epstein Barr viruses. The recoveries of spiked human urine samples at 0.30-0.50 ng mL-1 concentration levels of gB-HCMV ranged between 91 to 105%. The proposed mpEIA method was vali...Continue Reading

Citations

Dec 19, 2019·ACS Sensors·Alejandra Alba-PatiñoRoberto de la Rica

❮ Previous
Next ❯

Related Concepts

Related Feeds

Related Papers

Nihon rinsho. Japanese journal of clinical medicine
T Yasuda
Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete
W Kempf, S Lautenschlager
The West Virginia Medical Journal
J L Neely
Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete
W Kempf, S Lautenschlager
© 2022 Meta ULC. All rights reserved