A rapid method for chemical fingerprint analysis of Hoodia species, related genera, and dietary supplements using UPLC-UV-MS

Journal of Pharmaceutical and Biomedical Analysis
Bharathi AvulaIkhlas A Khan

Abstract

Recently, ultra-performance liquid chromatography (UPLC) has proven to be one of the most promising developments in the area of high-speed chromatographic separations with increased sensitivity and resolution. In this work, a reverse phase chromatographic method was developed using UPLC for the chemical fingerprint analysis of 12 hoodigosides, related genera and dietary supplements. The method is also used for the quantification of P57 in Hoodia species and dietary supplements that claim to contain Hoodia. The analysis was performed on a Waters Acquity UPLC system with an Acquity UPLC BEH C18 column (100 mm x 2.1mm I.D., 1.7 microm) and a gradient elution of water and acetonitrile, both containing 0.05% formic acid with a run time of 15 min. The calibration curve of P57 showed good linearity (r(2)>0.999) within the established range (1-100 microg/mL). The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.3 and 0.9 microg/mL, respectively. The RSD for intra- and inter-day were less than 3.0%, and the recovery efficiency as 97-103%. LC-mass spectrometry coupled with electrospray ionization (ESI) interface method is described for the identification of P57. The developed method was successfully applied t...Continue Reading

References

Sep 24, 2004·Rapid Communications in Mass Spectrometry : RCM·Robert PlumbAndrew Wright
Jul 3, 2007·Phytochemistry·Fanie R van HeerdenPhilip J Gunning
Sep 5, 2007·Steroids·Rahul S PawarIkhlas A Khan
Nov 22, 2007·Journal of Pharmaceutical and Biomedical Analysis·Vaijanath G DongreAshok Kumar

❮ Previous
Next ❯

Citations

Sep 28, 2010·Current Opinion in Investigational Drugs·Brittany L GrafDavid M Ribnicky
Nov 26, 2013·Chromatographia·Judyta Cielecka-PiontekPiotr Garbacki
Jul 15, 2015·Epilepsy & Behavior : E&B·Eva-Maria Pferschy-Wenzig, Rudolf Bauer
Jul 20, 2013·Journal of Forensic Sciences·Gerard GathierBarbara Gravendeel
Sep 19, 2015·BMC Complementary and Alternative Medicine·Wei HuXing Y Meng
Jan 10, 2013·Journal of Pharmaceutical and Biomedical Analysis·Xuesong LiuYongjiang Wu
Jan 26, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Barry LynchPalma Ann Marone
Mar 15, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Barry LynchPalma Ann Marone
Dec 10, 2008·Journal of Separation Science·Chidananda Swamy RumallaIkhlas A Khan
Dec 25, 2009·Clinical Pharmacology and Therapeutics·T J Smillie, I A Khan
Dec 16, 2017·Food & Function·Krassimir P VelikovSergey M Melnikov

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.