A rapid method for detecting bacterial contamination in the presence of Penicillium and Streptomyces antibiotic fermentations

Biotechnology and Bioengineering
J McLaughlinT Forrest

Abstract

Contamination in antibiotic fermentation processes results in major economic and process problems. The detection and elimination of contamination is a continual objective for fermentation companies. While efforts continue to eliminate contamination by improving equipment and sterile techniques, it is still imperative to have a rapid method for detecting contamination in laboratory-stage inoculum and seed tanks. This article describes the successful studies leading to the adoption of the BACTEC, an automatic bacterial detection system, as a supplemental detection technique. The BACTEC system detects contamination by incubating samples with a selected(14)C-labeled substrate or substrates. The resulting metabolism of substrate produces (14)C-labeled CO(2) which is then quantified and expressed as a growth index, permitting detection of contamination more rapidly at a much earlier time than is possible with conventional detection techniques that involve Phenol red dextrose broth, streak plates, and microscopic examination techniques.

Citations

Jan 15, 1986·Biochimica Et Biophysica Acta·E S David, M M Crerar
Nov 8, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Irene O L McHugh, Ayly Ling Tucker

Related Concepts

Antibiotics
Bone Plates
Fermentation
Metabolism
Microscopy
Penicillium
Phenolsulfonphthalein
Problem
Streptomyces
Laboratory Studies

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