PMID: 6406770Apr 1, 1983Paper

A rapid method for detecting inhibitors of both cyclo-oxygenase lipoxygenase metabolites of arachidonic acid

Journal of Pharmacological Methods
J Harvey, D J Osborne

Abstract

A method for detecting inhibitors of arachidonic acid metabolism is described. Guinea pig peritoneal polymorphonuclear leukocytes incubated with [14C] arachidonic acid were stimulated with calcium ionophore A23187. The radiolabelled products were identified by HPLC and GC/MS as cyclo-oxygenase and lipoxygenase metabolites. A TLC system was utilized to separate these metabolites which were then analysed by automatic quantitative scanning. This method allowed rapid determination of the radiolabel incorporated into each metabolite. This method of analysis was used to study the actions of indomethacin, NDGA, benoxaprofen, and BW775C on the formation of radiolabelled arachidonic acid metabolites.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R C MurphyB Samuelsson
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Dec 1, 1977·Biomedical Mass Spectrometry·A F CockerillW Dawson
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Jan 1, 1981·Naunyn-Schmiedeberg's Archives of Pharmacology·K BruneB A Peskar

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Citations

Mar 1, 1987·Prostaglandins, Leukotrienes, and Medicine·G H RaoJ G White
Sep 1, 1987·Kidney International·K D GardnerA A Leon
May 1, 1986·The British Journal of Dermatology·P D MauriceP C Bather
May 1, 1984·The Journal of Pharmacy and Pharmacology·E A Kitchen, W Dawson

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