PMID: 1795Dec 1, 1975

A rapid method for measuring guanylate cyclase activity in mammary tissue

Proceedings of the Society for Experimental Biology and Medicine
R D Smith, J A Rillema


A simple and rapid method for measuring guanylate cyclase activity in broken cell preparations of biological tissues is described. This method employs the rate of conversion of [32P]GTP to [32P]cyclic-GMP. The product of this reaction is isolated by ion-exchange chromatography and by a ZnSO4-Ba(OH)2 precipitation at pH 5.7. Using this method, about 30-50 samples can be assayed for guanylate cyclase activity during a 5-6 hr period. The characteristics of this enzyme in the mammary gland were found to be similar to those described for other tissues using different methods for measuring guanylate cyclase activity.


Sep 28, 1984·Biochemical and Biophysical Research Communications·D L Vesely

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