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A rapid method for removal of detergents from protein solution

Analytical Biochemistry

Apr 15, 1983

T Horigome, H Sugano

PMID: 6869826

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Abstract

A simple and rapid technique is described for the removal of Triton X-100, deoxycholate, and cholate from protein solutions. The method involves a 2-min centrifugation of the sample on a Bio-Beads SM-2 bed prepared in a microcentrifuge tube and is suitable for multiple assays of 0.05- t...read more

Mentioned in this Paper

Deoxycholic Acid, Monoammonium Salt
Gene Products, Protein
Solutions
Cholic Acids
Cholic Acid
Ultracentrifugation
Vigilon
Tensides
Synthetic Detergent
Triton X-305
Paper Details
References
  • References4
  • Citations22
1
  • References4
  • Citations22
123

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A simple procedure for removal of Triton X-100 from protein samples

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A rapid method for removal of detergents from protein solution

Analytical Biochemistry

Apr 15, 1983

T Horigome, H Sugano

PMID: 6869826

DOI:

Abstract

A simple and rapid technique is described for the removal of Triton X-100, deoxycholate, and cholate from protein solutions. The method involves a 2-min centrifugation of the sample on a Bio-Beads SM-2 bed prepared in a microcentrifuge tube and is suitable for multiple assays of 0.05- t...read more

Mentioned in this Paper

Deoxycholic Acid, Monoammonium Salt
Gene Products, Protein
Solutions
Cholic Acids
Cholic Acid
Ultracentrifugation
Vigilon
Tensides
Synthetic Detergent
Triton X-305

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Related Papers

Analytical Biochemistry

A simple procedure for removal of Triton X-100 from protein samples

Analytical BiochemistryMay 1, 1973
P W Holloway
Biochemical and Biophysical Research Communications

Production of a monoclonal antibody directed against rat liver glutathione-insulin transhydrogenase

Biochemical and Biophysical Research CommunicationsDecember 14, 1984
D B DawsonP T Varandani
Paper Details
References
  • References4
  • Citations22
1
  • References4
  • Citations22
123

Download from

PubMed
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