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A rapid method for the assay of guanylate cyclase

Journal of Cyclic Nucleotide Research

Jan 1, 1975

Govind S R Krishna, Nagasathiya Krishnan

PMID: 5469

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Abstract

An extremely rapid and sensitive assay for guanylate cyclase utilizing [alpha-32P]-GTP has been developed. It involves incubation of 5-100 mug of enzyme protein with 1 mM [alpha-32P]-GTP in 40 mM Tris HC1 buffer (pH 7.4) containing 3-3 mM MnSO2, 10 mM theophylline and 1 mM cyclic GMP. T...read more

Mentioned in this Paper

Ion-Exchange Chromatography Procedure
Tissue Specificity
Microchemistry
Brain
Lung
Phosphorus Radioisotopes
Kidney
Guanosine Triphosphate
Myocardium
Adrenal Glands
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A rapid method for the assay of guanylate cyclase

Journal of Cyclic Nucleotide Research

Jan 1, 1975

Govind S R Krishna, Nagasathiya Krishnan

PMID: 5469

DOI:

Abstract

An extremely rapid and sensitive assay for guanylate cyclase utilizing [alpha-32P]-GTP has been developed. It involves incubation of 5-100 mug of enzyme protein with 1 mM [alpha-32P]-GTP in 40 mM Tris HC1 buffer (pH 7.4) containing 3-3 mM MnSO2, 10 mM theophylline and 1 mM cyclic GMP. T...read more

Mentioned in this Paper

Ion-Exchange Chromatography Procedure
Tissue Specificity
Microchemistry
Brain
Lung

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