PMID: 6161193Jan 1, 1980Paper

A rapid method for the detection of antibodies to cell surface antigens: a solid phase radioimmunoassay using cell membranes

Journal of Immunological Methods
F D HowardL A Herzenberg

Abstract

Cell membranes isolated from murine lymphocytes or ascites tumors bind tightly to the surface of flexible plastic microtiter plates in the absence of additional proteins. This allows the detection of membrane associated molecules by specific antibodies and thus forms the basis for a rapid and sensitive radioimmunoassay for antibodies to membrane-bound components. The assay compares favorably with a variety of methods currently used to detect antibodies to cell surface antigens. The assay detects a variety of well characterized murine cell surface antigens (H-2, I-A, T-200, Thy-1.2, Ig). The level of antibody binding to membranes on plates correlates well with antigen density on intact cells. A modification of the assay involving competition between cross-reacting antibodies allows detection and resolution of closely spaced antigenic determinants.

References

Jun 30, 1975·Annals of the New York Academy of Sciences·M R Loken, L A Herzenber
Jan 1, 1978·Journal of Immunological Methods·J W Goding
Jan 1, 1979·Immunological Reviews·J A Ledbetter, L A Herzenberg
Mar 1, 1972·The Review of Scientific Instruments·W A BonnerL A Herzenberg

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Citations

Jan 1, 1981·Archives of Dermatological Research·A R Ahmed, R Murahata
Jan 1, 1995·Archives of Virology·J H Broughan, W H Wunner
Dec 17, 1985·Journal of Immunological Methods·M LiebertT R Hakala
Feb 11, 1987·Journal of Immunological Methods·H BaumgartenO Götze
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A E FrankelL A Herzenberg
Jan 1, 1985·Differentiation; Research in Biological Diversity·A Kindler-RöhrbornM F Rajewsky
Feb 22, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society· RamandeepM Raje
May 23, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Ramandeep KaurManoj Raje
Nov 1, 1990·Journal of Dairy Science·K M KuzmanoffC W Beattie
Oct 1, 1990·Journal of Dairy Science·K M KuzmanoffC W Beattie
Nov 1, 1991·Journal of Dairy Science·K M Kuzmanoff, C W Beattie
Dec 1, 1985·Journal of Surgical Oncology·M BhattacharyaJ J Barlow
May 26, 1983·Biochimica Et Biophysica Acta·T MaedaS Q Mehdi
Sep 13, 1982·Biochimica Et Biophysica Acta·K BalakrishnanH M McConnell

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