PMID: 6540598Jun 5, 1984Paper

A rapid method of reconstituting human erythrocyte sugar transport proteins

Biochemistry
A Carruthers, D L Melchior

Abstract

A rapid reconstitution procedure for human erythrocyte hexose transfer activity is described. The procedure (reverse-phase evaporation) avoids exposure of the isolated proteins to detergent, organic solvent, sonication, or freeze-thaw steps during insertion into synthetic membranes and may be effected within 15 min. The so-formed vesicles are unilamellar structures with a large encapsulated volume, narrow size range, and low passive permeabilities. Contamination by carry-through of endogenous (red cell) lipids is less than 1%. Reconstituted hexose transfer activity was examined by using unfractionated proteins (bands 3, 4.5, and 6) and purified proteins (bands 4.5 and 3). With unfractionated proteins, hexose transport activity is low [0.34 mumol X (mg of protein)-1 X min-1], is inhibited by cytochalasin B, and increases monotonically with protein concentration. Kinetic analysis indicates that Vmax values for both influx and efflux of D-glucose are identical. Reconstitution of the cytochalasin B binding protein (band 4.5) results in hexose transport with high specific activity [5 mumol X (mg of protein)-1 X min-1] and symmetry in transfer kinetics. Band 3 proteins also appear to mediate cytochalasin B sensitive D-glucose transpo...Continue Reading

References

May 1, 1973·Analytical Biochemistry·P W Holloway
Aug 1, 1973·Analytical Biochemistry·H S Garewal
Aug 1, 1965·Journal of Molecular Biology·A D BanghamJ C Watkins
Feb 1, 1983·Biochimica Et Biophysica Acta·A Carruthers, D L Melchior
Aug 24, 1983·Biochimica Et Biophysica Acta·R L Shelton, R G Langdon
Jan 1, 1980·Annual Review of Biophysics and Bioengineering·F Szoka, D Papahadjopoulos
Jun 16, 1981·Biochimica Et Biophysica Acta·M N Jones, J K Nickson
Aug 1, 1961·The Biochemical Journal·G N WILKINSON

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Citations

Jan 16, 1998·Applied Biochemistry and Biotechnology·C Neumann-SpallartT Schalkhammer
Jul 25, 1985·Biochimica Et Biophysica Acta·D M MalchoffR G Langdon
Sep 15, 1988·Biochimica Et Biophysica Acta·D M OjciusA K Solomon
Oct 16, 1989·Biochimica Et Biophysica Acta·S NaderiD L Melchior
Jan 1, 1988·The International Journal of Biochemistry·T D Madden
Jan 1, 1994·Neurobiology of Aging·J W PettegrewL R Muenz
Apr 27, 1987·Biochimica Et Biophysica Acta·M K Jain, D Zakim
Oct 11, 1988·Biochimica Et Biophysica Acta·D L Baly, R Horuk
Oct 27, 1997·Journal of Pharmacological and Toxicological Methods·W M Li, J H McNeill
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C C ChenK J Isselbacher
Jul 20, 2007·The Journal of General Physiology·David M BlodgettAnthony Carruthers
May 24, 1995·Biochimica Et Biophysica Acta·S NaderiD L Melchior
Jun 1, 2020·Pflügers Archiv : European journal of physiology·Hélène Guizouarn, Benoit Allegrini
Sep 6, 1985·Science·M MuecklerH F Lodish

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