A rapid microtiter plate method to measure carbon dioxide evolved from carbon substrate amendments so as to determine the physiological profiles of soil microbial communities by using whole soil.

Applied and Environmental Microbiology
Colin D CampbellJacqueline M Potts

Abstract

Sole-carbon-source tests (Biolog), designed to identify bacteria, have become very popular for metabolically fingerprinting soil microbial communities, despite disadvantages associated with the use of carbon source profiles that primarily select for fast-growing bacteria. In this paper we describe the use of an alternative method that combines the advantages of the Biolog community-level physiological profile (CLPP) method, in which microtiter-based detection plates are used, with the ability to measure carbon dioxide evolution from whole soil. This method facilitates measurement over short periods of time (4 to 6 h) and does not require the extraction and culturing of organisms. Deep-well microtiter plates are used as test wells into which soil is placed. The apparatus to fill the deep-well plates and interface it with a second removable detection plate is described. Two detection systems, a simple colorimetric reaction in absorbent alkali and scintillation counting with radioactive carbon sources, are described. The methods were compared to the Biolog-CLPP system by using soils under different vegetation types and soil treated with wastewater sludge. We aimed to test the hypothesis that using whole soil would have specific ad...Continue Reading

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Citations

May 11, 2011·Microbial Ecology·Evagelia S PapadopoulouUrania Menkissoglu-Spiroudi
Sep 6, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·M G KibblewhiteM J Swift
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Mar 29, 2014·Ecotoxicology·Stanislav Pen-MouratovYosef Steinberger
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Nov 12, 2011·Ecological Applications : a Publication of the Ecological Society of America·Pablo García-PalaciosAdrián Escudero
Sep 29, 2011·The Science of the Total Environment·J J C DawsonM I Stutter
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Sep 28, 2015·The Science of the Total Environment·Sebastian HupfaufBettina Eichler-Löbermann
Sep 29, 2015·Environment International·Kristian K BrandtEdward Topp
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Aug 4, 2015·Environmental Science and Pollution Research International·Annette BérardYvan Capowiez
Sep 20, 2005·Environmental Pollution·Karin KaufmannPatrick Höhener

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