PMID: 6405660Mar 1, 1983Paper

A rapid one-step purification of NADPH-cytochrome c (P-450) reductase from rat liver microsomes

Analytical Biochemistry
E A ShephardI R Phillips

Abstract

NADPH-cytochrome c (P-450) reductase from liver microsomes of phenobarbital-treated rats has been purified in a single step by affinity chromatography on agarose-hexane-adenosine 2',5'-diphosphate. As determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, enzyme assay, and radioimmunoassay the protein obtained by this single step procedure is as pure as that isolated by multicolumn procedures.

References

Oct 23, 1974·Biochemical and Biophysical Research Communications·J L Vermilion, M J Coon
Jan 1, 1967·Advances in Cancer Research·H V Gelboin

❮ Previous
Next ❯

Citations

Jun 1, 1988·Biochemical Pharmacology·C A AustinI R Phillips
Jan 1, 1989·Biochemical Pharmacology·D W Starke, J J Mieyal
Nov 1, 1986·Journal of Steroid Biochemistry·D HendersonM F el Etreby
Oct 1, 1986·Archives of Gerontology and Geriatrics·C Santa Maria, A Machado
Jan 1, 1983·Biotechnology Advances·T BeckerS E Builder
Jul 1, 1986·The Histochemical Journal·C J Van Noorden, R G Butcher
Apr 6, 2004·Biochimica Et Biophysica Acta·Marat B MuratalievF Ann Walker
Apr 22, 1993·Biochemical Pharmacology·K E ThummelJ T Slattery
Apr 16, 2003·Molecular Pharmacology·Brian A CarrGarold S Yost
Feb 28, 1986·Journal of Chromatography·H J Marriage, D J Harvey
Aug 15, 1989·Archives of Biochemistry and Biophysics·H YamadaH Yoshimura
May 31, 1991·Biochemical and Biophysical Research Communications·I BenvenisteF Durst
Jan 14, 1986·Biochemical and Biophysical Research Communications·J J Lee, L S Kaminsky

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.